Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3397891..3398511 Replicon chromosome
Accession NZ_CP050078
Organism Citrobacter koseri strain NJ

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag NCK_RS16075 Protein ID WP_002892050.1
Coordinates 3398293..3398511 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag NCK_RS16070 Protein ID WP_012133514.1
Coordinates 3397891..3398265 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCK_RS16060 (3393027) 3393027..3394220 + 1194 WP_047458420.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
NCK_RS16065 (3394243) 3394243..3397389 + 3147 WP_012133513.1 efflux RND transporter permease AcrB -
NCK_RS16070 (3397891) 3397891..3398265 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
NCK_RS16075 (3398293) 3398293..3398511 + 219 WP_002892050.1 HHA domain-containing protein Toxin
NCK_RS16080 (3398695) 3398695..3399246 + 552 WP_219935846.1 maltose O-acetyltransferase -
NCK_RS16085 (3399360) 3399360..3399830 + 471 WP_024130587.1 YlaC family protein -
NCK_RS16090 (3399908) 3399908..3400048 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NCK_RS16095 (3400050) 3400050..3400310 - 261 WP_012133519.1 type B 50S ribosomal protein L31 -
NCK_RS16100 (3400536) 3400536..3402086 + 1551 WP_058668708.1 EAL domain-containing protein -
NCK_RS16105 (3402126) 3402126..3402479 - 354 WP_012133521.1 DUF1428 family protein -
NCK_RS16110 (3402559) 3402559..3403173 - 615 WP_104868983.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3397891..3405935 8044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T152223 WP_002892050.1 NZ_CP050078:3398293-3398511 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T152223 NZ_CP066057:c1525683-1525462 [Moraxella nonliquefaciens]
TTGCTTTGTCCTTATTCCGATACTTTATATGACTCAAAATTTAACAACATGAAAGAATTGCGTATCCAAATCGGGGGTGA
CCCTTATCGTGCTTTTTATACGTTTGACCCCATACGACAAGCGATTGTGCTATGTGCAGGCAACAAAGTGGGCAATGAAA
AGCAGTTTTATAAACAGATGATACCGCTTGCTGATGAGTTTACATCAGCAGCATTTGGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT152223 WP_012133514.1 NZ_CP050078:3397891-3398265 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT152223 NZ_CP066057:c1525440-1525141 [Moraxella nonliquefaciens]
ATGGCAAATCTTAATGATATGCTCGCCAAATCACCAAAAGAGCGTTTGGCTCGTGTGCAAAAAATGGCAGATGAGATGAT
TTTGGATTACAAGCTTTATCAGTTGCGTGAAGAGTTGGAGCTGTCGCAAAAACAGCTTGCCGAAGCCATGGGCATTACCC
AGCCGACCTTATCCGCCATTGAGAACCGCGGGGCAGAGATTAAGCTGTCTACACTAAAATGCTATGTAGAGACCATGGGT
GGAAAATTACGCATTGATGTGGAATTACCAACAGGTAAGCACGTTGGGTTTAATATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References