Detailed information of TA system TA152221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2301148..2301787
• Replicon: chromosome
• Accession: NZ_CP050078
• Organism: Citrobacter koseri strain NJ

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A381H3Z1
• Locus tag: NCK_RS10860
• Protein ID: WP_071818852.1
• Coordinates: 2301148..2301324 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: NCK_RS10865
• Protein ID: WP_012132350.1
• Coordinates: 2301371..2301787 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCK_RS10840 (2296242)	2296242..2297117	+	876	WP_012132344.1	AraC family transcriptional regulator	-
NCK_RS10845 (2297094)	2297094..2298260	-	1167	WP_112002075.1	benzoate/H(+) symporter BenE family transporter	-
NCK_RS10850 (2298352)	2298352..2298888	+	537	WP_058669186.1	XRE family transcriptional regulator	-
NCK_RS10855 (2298967)	2298967..2300928	+	1962	WP_219937057.1	U32 family peptidase	-
NCK_RS10860 (2301148)	2301148..2301324	+	177	WP_071818852.1	type II toxin-antitoxin system HicA family toxin	Toxin
NCK_RS10865 (2301371)	2301371..2301787	+	417	WP_012132350.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NCK_RS10870 (2301839)	2301839..2303248	+	1410	WP_047463638.1	PLP-dependent aminotransferase family protein	-
NCK_RS10875 (2303569)	2303569..2304993	+	1425	WP_012132353.1	aminobutyraldehyde dehydrogenase	-
NCK_RS10880 (2305092)	2305092..2305187	-	96	WP_071818853.1	stress response membrane protein YncL	-
NCK_RS10885 (2305379)	2305379..2305552	+	174	WP_049008504.1	GhoT/OrtT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6810.81 Da
• Isoelectric Point: 10.8122

>T152221 WP_071818852.1 NZ_CP050078:2301148-2301324 [Citrobacter koseri]
VKQSEFRRWLESQGVDVTNGTNHLKLRYHDKRSVMPRHPGDEIKDTLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T152221 NZ_CP066057:c574850-574521 [Moraxella nonliquefaciens]
TTGACCTTTATTGAAACCAGTATTTTTACAAAAGCCATTAAAGAATTATTTGATGATGATGAATATCGCAAGATTCAAAA
TGAGCTGTTAAATGACCCACAAAAAGGGGATTTAATTCAAGGTACTGGTGGACTTAGAAAGATTCGTTTTGGTCGTAACA
GTGGCAAGAGTAGCGGAGTTAGAATCTTATATTATTATACAGATGAATTTGGTCGCATTTATCTGTTTGCGGTTTATCCA
AAATCCGTCAAAGATAATATTACTAATGCTGAAAAAGCGGTTTTTAAAACCCTTATCACCATACTAAAACAGGAGCTTAA
AAATGGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15342.61 Da
• Isoelectric Point: 4.4377

>AT152221 WP_012132350.1 NZ_CP050078:2301371-2301787 [Citrobacter koseri]
MRYPVNLIPAEEGGYVVSFPDIPEALTQGDTRHESLEAAQGALVTAFEFYFEDNQPIPLPSMVDEADDYVEIPLSIASKV
LLLNAFLESKITQQELARRIGRPKQEITRLFDLKHATKIDAVQIAAKALGKELSLTML

Nucleotide

• Length: 417 bp

>AT152221 NZ_CP066057:c574528-574247 [Moraxella nonliquefaciens]
ATGGATAAGCAACTATTTGATGACATCGTCGCTTCTATGAATGAAGCCATTGCCATTACAAAGGGCGAAATTGCCCCAAG
TCGTACATTCACCTATGAACGCCCAAACATCAAAGACATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGTTACATATTAGCCCTAAAACGCTTAGAAATTGGGAACAAGGCATTAGAACACCAACAGGCCCAGCCATTACCCTAATT
AGATTGCTTGACAAAAACCCTGATTTAATTAGCATGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A381H3Z1