Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 835729..836383 Replicon chromosome
Accession NZ_CP050078
Organism Citrobacter koseri strain NJ

Toxin (Protein)


Gene name cptA Uniprot ID A0A078LNB4
Locus tag NCK_RS04005 Protein ID WP_024130921.1
Coordinates 835976..836383 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A078LG12
Locus tag NCK_RS04000 Protein ID WP_024130922.1
Coordinates 835729..835995 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCK_RS03975 (830870) 830870..832303 - 1434 WP_012135007.1 6-phospho-beta-glucosidase BglA -
NCK_RS03980 (832424) 832424..833152 - 729 WP_049008977.1 MurR/RpiR family transcriptional regulator -
NCK_RS03985 (833205) 833205..833516 + 312 WP_047461422.1 N(4)-acetylcytidine aminohydrolase -
NCK_RS03990 (833678) 833678..834337 + 660 WP_012135004.1 hemolysin III family protein -
NCK_RS03995 (834492) 834492..835472 - 981 WP_047461425.1 tRNA-modifying protein YgfZ -
NCK_RS04000 (835729) 835729..835995 + 267 WP_024130922.1 FAD assembly factor SdhE Antitoxin
NCK_RS04005 (835976) 835976..836383 + 408 WP_024130921.1 protein YgfX Toxin
NCK_RS04010 (836481) 836481..837005 - 525 WP_012134999.1 flavodoxin FldB -
NCK_RS04015 (837109) 837109..838005 + 897 WP_047461431.1 site-specific tyrosine recombinase XerD -
NCK_RS04020 (838029) 838029..838739 + 711 WP_047461435.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NCK_RS04025 (838745) 838745..840478 + 1734 WP_047461438.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15888.77 Da        Isoelectric Point: 11.2845

>T152210 WP_024130921.1 NZ_CP050078:835976-836383 [Citrobacter koseri]
VVLWQSDLRVSWRAQWLSLLIHGLVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRINTRQGEIKLLMDGRLRWQGQ
DWTIQGPPWMLKTGMMLRLRADSGKRQHLWLAADSMDDAEWRDLRRLMLQQATQG

Download         Length: 408 bp

>T152210 NZ_CP066042:c77788-77426 [Staphylococcus saccharolyticus]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTCCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTTGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACAGTAATTGTTGCTGCAATTACAGGCAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAATACAAATTAGACAAGGACTCCGTTATATTGCTTGAACAGATTAGA
ACATTAGATAAGAAGCGTTTAAAAGAAAAGCTGACTTATTTATCTGAAAGCAAGATGAAAGAAGTTGATAACGCGCTAGA
CATTAGTCTAGGTCTACATAACTTTGATCACCAAAAATCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10476.00 Da        Isoelectric Point: 4.8836

>AT152210 WP_024130922.1 NZ_CP050078:835729-835995 [Citrobacter koseri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDNLSDEEKCIFVRLLECDDPDLFNWLMNHGKPGDAELEQMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT152210 NZ_CP066042:c77955-77785 [Staphylococcus saccharolyticus]
ATGTTATCTTTTAATCAAACTAGAACTAACAGTCTTGAACAATCGTTGAAAGAGGGCTATTCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACGGAAGCTTTTCCTATTGAATGTGAAGCTTGCGATTGTAATGAAACATATTTGACTTCTAATTCAC
AAAGTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A078LNB4

Antitoxin

Source ID Structure
AlphaFold DB A0A078LG12

References