Detailed information of TA system TA152209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 293498..294084
• Replicon: chromosome
• Accession: NZ_CP050078
• Organism: Citrobacter koseri strain NJ

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: NCK_RS01335
• Protein ID: WP_200024239.1
• Coordinates: 293716..294084 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: NCK_RS01330
• Protein ID: WP_047460592.1
• Coordinates: 293498..293719 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCK_RS01310 (289664)	289664..290590	+	927	WP_012135564.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
NCK_RS01315 (290587)	290587..291864	+	1278	WP_012135563.1	branched chain amino acid ABC transporter permease LivM	-
NCK_RS01320 (291861)	291861..292628	+	768	WP_047460588.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
NCK_RS01325 (292646)	292646..293359	+	714	WP_047463875.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
NCK_RS01330 (293498)	293498..293719	+	222	WP_047460592.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
NCK_RS01335 (293716)	293716..294084	+	369	WP_200024239.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
NCK_RS01340 (294285)	294285..295601	+	1317	WP_024131025.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
NCK_RS01345 (295702)	295702..296589	+	888	WP_024131024.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
NCK_RS01350 (296586)	296586..297431	+	846	WP_047460601.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
NCK_RS01355 (297440)	297440..298510	+	1071	WP_012135556.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	288493..299256	10763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13858.19 Da
• Isoelectric Point: 7.3169

>T152209 WP_200024239.1 NZ_CP050078:293716-294084 [Citrobacter koseri]
MTLQFISAEEIIRFHDKLLSVTPGVPGMPDPGRVEALLYRVLNKYEYESITDVWILAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIFLAANPDYVDMTVEAAAGRLSLEEIVQRLRR

Nucleotide

• Length: 369 bp

>T152209 NZ_CP066041:c668053-667799 [Streptococcus oralis]
ATGCTGCTCAAGTTTACAGAAGATGCCTGGGCGGATTATTGCTATTGGCAAAACCAAGATAAGAAAACATTAAAAAGAAT
CAATAAATTAATCAAGGATATTCAACGCGATCCTTTTGTAGGAATGGGGAAACCAGAACCGCTCAAGTATGATTATCAAG
GAGCTTGGTCGCGGCGCATTGATGCAGAAAATCGCTTGATTTATATGATAGATGGAGATAGCGTGGCTTTCTTGTCCTTC
AAAGATCATTACTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8113.10 Da
• Isoelectric Point: 5.0495

>AT152209 WP_047460592.1 NZ_CP050078:293498-293719 [Citrobacter koseri]
MRTMNYSEVRQNLASVLDSAATGTPVTITRRGHKSAVIISAEEFERYQTAKLDAEFDAIMGVHGDELRELADK

Nucleotide

• Length: 222 bp

>AT152209 NZ_CP066041:c668311-668057 [Streptococcus oralis]
ATGGAAGTGGTCCTTTACTCAACATTCAGAAATCATTTAAAAGACTACATGAAGAAGGTAAACGATGAATTTGAGCCTTT
AACGGTGGTCAATAAAAATCCAGATGAGGATATTGTAGTCCTTTCAAAGAGCGAGTGGGATAGTATTCAGGAAACCCTGA
GAATCGCTCAAAATAAGGAACTTTCTGACAAGGTCTTGCGAGGAATGGCTCAAGTTCGTGCTGGAAGCACTCAGGTGCAT
GTGATTGAGGAGTGA