Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21068..21711 Replicon plasmid p070_A-KPC-2
Accession NZ_CP050075
Organism Enterobacter kobei strain 070

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HA514_RS24465 Protein ID WP_001044770.1
Coordinates 21068..21484 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HA514_RS24470 Protein ID WP_001261282.1
Coordinates 21481..21711 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA514_RS24430 16610..16846 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
HA514_RS24435 16843..17205 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
HA514_RS24440 17223..18917 - 1695 WP_000105636.1 mercury(II) reductase -
HA514_RS24445 18969..19391 - 423 WP_001340589.1 organomercurial transporter MerC -
HA514_RS24450 19427..19537 - 111 Protein_22 mercuric transport protein periplasmic component -
HA514_RS24455 19553..20250 - 698 Protein_23 IS1 family transposase -
HA514_RS24460 20284..20994 - 711 WP_004152334.1 ATP-binding protein -
HA514_RS24465 21068..21484 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HA514_RS24470 21481..21711 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HA514_RS24475 21668..22129 + 462 WP_072093212.1 hypothetical protein -
HA514_RS24480 22364..22570 + 207 WP_004153649.1 hypothetical protein -
HA514_RS24485 22616..22924 + 309 WP_004152336.1 hypothetical protein -
HA514_RS24490 22952..23281 + 330 WP_004152337.1 hypothetical protein -
HA514_RS24495 23307..23705 + 399 WP_004171440.1 hypothetical protein -
HA514_RS24500 23712..24044 + 333 WP_004152339.1 hypothetical protein -
HA514_RS24505 24044..24826 + 783 WP_004152340.1 site-specific integrase -
HA514_RS24510 25718..25948 - 231 WP_011977773.1 hypothetical protein -
HA514_RS24515 26040..26502 - 463 Protein_35 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..109956 109956
- flank IS/Tn - - 26622..27941 1319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T152208 WP_001044770.1 NZ_CP050075:c21484-21068 [Enterobacter kobei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152208 NZ_CP066041:c639695-639330 [Streptococcus oralis]
GTGCATACAATTTACTTCTATAAGGACAAAAATGGAAATGAGCTAGTCTTAGATTATATGAGAGAATTGGCTAGAAAGAA
GAGTAAGGACAGTCGCATCAAACTCAATAAACTAAACGACTATATCGAGTTGCTTAGTCAGCATGGGACTAGAACTGGTG
AACCCTATATCAAACATTTGGAAGATGAGATTTGGGAACTACGACCTCTTAAGGATAGGATATTATTTGTAGCATGGGTT
GATGGGAGTTTTGTTCTTTTACATCATTTTGTTAAAAAGACTCAGAAAACTCCTAGGAGAGAGATTGATAAAGCCAAGCG
TGAACTGAAAGACATAAAAGAAAGAGGGTTAAGTGATGAAGAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT152208 WP_001261282.1 NZ_CP050075:c21711-21481 [Enterobacter kobei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT152208 NZ_CP066041:c639340-639047 [Streptococcus oralis]
ATGAAGAATAGTGCCATTGGAAGTAATTGGAAGGATATCCGATCTGAACTCTTTACCAAGGAGGAAATCCTTGAAAGTGA
TATGCGAGTGGCTATCATGAGTGAGTTGATTGAGGCTAGACATGAGCAAGGTATCAGTCAGAAAAAGCTAGAGGAACTCA
GTGGAGTAAGCCAGCCTGTTATAGCTAGGATGGAGACAGGAAAGACCAGTCCTCAGTTGGATACGGTCTTAAAAGTTTTA
GCAAGTTTAGGAAAGACACTAGCAGTCGTTCCACTTGAACAGGAAAAAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References