Detailed information of TA system TA152207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 71828..72353
• Replicon: plasmid p070
• Accession: NZ_CP050074
• Organism: Enterobacter kobei strain 070

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9GHC5
• Locus tag: HA514_RS24050
• Protein ID: WP_009309918.1
• Coordinates: 72048..72353 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HA514_RS24045
• Protein ID: WP_004197642.1
• Coordinates: 71828..72046 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA514_RS24025	66959..68602	+	1644	WP_166479498.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
HA514_RS24030	68749..68913	+	165	Protein_70	LuxR family transcriptional regulator	-
HA514_RS24035	68958..69926	-	969	WP_004181748.1	IS5-like element IS903 family transposase	-
HA514_RS24040	69961..71205	+	1245	Protein_72	hypothetical protein	-
HA514_RS24045	71828..72046	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HA514_RS24050	72048..72353	+	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	Toxin
HA514_RS24055	72582..72722	+	141	WP_162898808.1	hypothetical protein	-
HA514_RS24060	72772..73107	+	336	WP_009309920.1	hypothetical protein	-
HA514_RS24065	73141..74157	+	1017	WP_009309921.1	hypothetical protein	-
HA514_RS24075	74355..75134	+	780	WP_023287113.1	site-specific integrase	-
HA514_RS24080	75192..75449	-	258	WP_009310077.1	hypothetical protein	-
HA514_RS24085	75578..75691	-	114	WP_014343462.1	hypothetical protein	-
HA514_RS24090	76223..77191	-	969	WP_072196505.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120547	120547
	-	flank	IS/Tn	-	-	68958..69926	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11571.24 Da
• Isoelectric Point: 6.4661

>T152207 WP_009309918.1 NZ_CP050074:72048-72353 [Enterobacter kobei]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T152207 NZ_CP066041:c539950-539762 [Streptococcus oralis]
ATGCCACTTACAGGTAAAGAAATGGTGAAACTAGCACTTGAGAATGGATGGGTTGAAGTTCGTCAAAGGGGTAGTCATCA
TCATTTTAAGAAAGAGGGTTTTTCATATCTTGTCACGATTCCAGTTCATGGAAATGAAGATTTGGGAAAGGGATTAGAAA
GAAAGATTCTCAAAGATTTAGGATTGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT152207 WP_004197642.1 NZ_CP050074:71828-72046 [Enterobacter kobei]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT152207 NZ_CP066041:c539703-539326 [Streptococcus oralis]
ATGTTAAAAACGTATCCAGCTATTTTTCATAAAGAAGGAACAGGATATTGGGTAGAATTCCCAGAATTTGGCGGTGGTAC
AGAAGGAAAAAATATCAATGAAGCGATGAAAAATGCTCGTGAAATGCTTGAAAATATTTTAGCTTCTTATATCGATGAAG
GGATGGCGCTGCCTACTCCAAGTGATATTTTAAAGATTGAAGTTTCAGATGGATTTGTATCAATGATTCAAGTTGATCCT
GCGCCCTTTGTGAGAAGTAACAAGGCGATTAGGAAGAATGTGACCGTTCCTGAATGGTTAGTTCGTCTTGCTGATCGAGA
AAAAATTAACTATTCAGAAGTTTTGGCGCAAGCGTTAGAAAAGAAGTTGCAAGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7H9GHC5