Detailed information of TA system TA152205

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4050738..4051531
• Replicon: chromosome
• Accession: NZ_CP050073
• Organism: Enterobacter kobei strain 070

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: HA514_RS19525
• Protein ID: WP_000854726.1
• Coordinates: 4051154..4051531 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: HA514_RS19520
• Protein ID: WP_001548930.1
• Coordinates: 4050738..4051064 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA514_RS19475	4046180..4047001	+	822	WP_008324532.1	DUF945 domain-containing protein	-
HA514_RS19480	4047218..4047919	+	702	WP_001548933.1	WYL domain-containing protein	-
HA514_RS19485	4047956..4048192	+	237	WP_001115854.1	hypothetical protein	-
HA514_RS19490	4048192..4048635	+	444	WP_097595467.1	hypothetical protein	-
HA514_RS19495	4048658..4049125	+	468	WP_001547765.1	hypothetical protein	-
HA514_RS19500	4049202..4049441	+	240	WP_097595469.1	DUF905 domain-containing protein	-
HA514_RS19505	4049539..4049997	+	459	WP_001548150.1	antirestriction protein	-
HA514_RS19510	4050013..4050489	+	477	WP_097595471.1	RadC family protein	-
HA514_RS19515	4050498..4050719	+	222	WP_000691994.1	DUF987 domain-containing protein	-
HA514_RS19520	4050738..4051064	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HA514_RS19525	4051154..4051531	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
HA514_RS19530	4051528..4052016	+	489	WP_001547769.1	hypothetical protein	-
HA514_RS19535	4052028..4052225	+	198	WP_000839269.1	DUF957 domain-containing protein	-
HA514_RS19540	4052310..4053152	+	843	WP_001548928.1	DUF4942 domain-containing protein	-
HA514_RS19545	4053339..4053866	-	528	WP_045134821.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T152205 WP_000854726.1 NZ_CP050073:4051154-4051531 [Enterobacter kobei]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T152205 NZ_CP066037:c1530364-1529942 [Burkholderia ambifaria]
GTGACGACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCATCCGCGGTGTTGTCCCGGTT
GCAGTCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCGATCACCTATGCCGAGATGCGATTCGGCGCAGTCGGCA
GAAAGGCTTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTTTCGCGGCTGGATGGCGTGCTGTCGTGGGATACCGCG
GCGGTCGATGCAACGACCGCGATTCGCGTCGACCTTGCCGCACGTGGCACGCCGATCGGCGCGAACGACGCGTCGATCGC
CGGTCACGCGATCGCGGCCGGTGCCGTGCTTGTAACGAACAACAGTCGCGAATTCAGCCGTGTCGCGGGGTTGTCGCTCG
AAGATTGGGCAGCCTCGGCCTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT152205 WP_001548930.1 NZ_CP050073:4050738-4051064 [Enterobacter kobei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT152205 NZ_CP066037:c1530627-1530361 [Burkholderia ambifaria]
ATGCGCACTGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGTGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGTTGCTGCTGCGTCCGGTGCGGCCGACGTGGCTGTCGTTCGCGACTGAAC
CACGGGCCGATGCCGACTTCCTCGTCGAGCGCCCGACGGTCGTCGAAGCCGGTCGTTTCGAGCTGCCCGGCGCCGATGAC
GATGCACAGACGGAGTCGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7