Detailed information of TA system TA152195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1605241..1606154
• Replicon: chromosome
• Accession: NZ_CP050073
• Organism: Enterobacter kobei strain 070

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HA514_RS07760
• Protein ID: WP_121527683.1
• Coordinates: 1605241..1605714 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J0PMR8
• Locus tag: HA514_RS07765
• Protein ID: WP_023337017.1
• Coordinates: 1605711..1606154 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA514_RS07740	1601409..1602710	+	1302	WP_121527679.1	type I-F CRISPR-associated protein Csy1	-
HA514_RS07745	1602707..1603642	+	936	WP_121527680.1	type I-F CRISPR-associated protein Csy2	-
HA514_RS07750	1603654..1604655	+	1002	WP_121527681.1	type I-F CRISPR-associated protein Csy3	-
HA514_RS07755	1604665..1605219	+	555	WP_121527682.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
HA514_RS07760	1605241..1605714	-	474	WP_121527683.1	RES family NAD+ phosphorylase	Toxin
HA514_RS07765	1605711..1606154	-	444	WP_023337017.1	DUF2384 domain-containing protein	Antitoxin
HA514_RS07770	1608152..1608370	-	219	WP_002211347.1	translation initiation factor IF-1	-
HA514_RS07775	1608655..1609359	-	705	WP_121527684.1	leucyl/phenylalanyl-tRNA--protein transferase	-
HA514_RS07780	1609405..1611126	-	1722	WP_166479256.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (15-147)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17584.89 Da
• Isoelectric Point: 5.4985

>T152195 WP_121527683.1 NZ_CP050073:c1605714-1605241 [Enterobacter kobei]
MIFYRLVTGRYASEAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVRKDTVLNQYQLFSIDIPDDQIDYLDKA
WLPEDWQENPAPVSTMDLGTGWLQAKSALALILPSCIIPYENNAILNPLHPSFHTALNSVQQLPFNFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T152195 NZ_CP066032:c3487997-3487803 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCAAAGTGTAACGGATGCGAATCCCGAACCGGCTATAGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16349.68 Da
• Isoelectric Point: 7.2062

>AT152195 WP_023337017.1 NZ_CP050073:c1606154-1605711 [Enterobacter kobei]
MRTWIPAQKPADNALWRYAGLPANRGMRLIEFLNQGLPVSVLDNIHEWTAMSKADILRVTGINERNVARRKSAGRTLTPD
ESERVARFVRVLDAAVDYFGSKEEAWNWLQAPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT152195 NZ_CP066032:3487626-3487804 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCAGTAATTTT

Structures

Antitoxin

• AlphaFold DB: A0A2J0PMR8