Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1082190..1082810 Replicon chromosome
Accession NZ_CP050073
Organism Enterobacter kobei strain 070

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag HA514_RS05150 Protein ID WP_014882802.1
Coordinates 1082190..1082408 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag HA514_RS05155 Protein ID WP_008499288.1
Coordinates 1082436..1082810 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA514_RS05120 1078199..1078459 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
HA514_RS05125 1078462..1078602 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HA514_RS05130 1078599..1079309 - 711 WP_121527529.1 GNAT family N-acetyltransferase -
HA514_RS05135 1079411..1080871 + 1461 WP_166479192.1 PLP-dependent aminotransferase family protein -
HA514_RS05140 1080843..1081310 - 468 WP_014882800.1 membrane protein -
HA514_RS05145 1081429..1081980 - 552 WP_014882801.1 maltose O-acetyltransferase -
HA514_RS05150 1082190..1082408 - 219 WP_014882802.1 hemolysin expression modulator Hha Toxin
HA514_RS05155 1082436..1082810 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
HA514_RS05160 1083322..1086468 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit -
HA514_RS05165 1086491..1087684 - 1194 WP_088126525.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T152193 WP_014882802.1 NZ_CP050073:c1082408-1082190 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T152193 NZ_CP066032:3270275-3270529 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT152193 WP_008499288.1 NZ_CP050073:c1082810-1082436 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT152193 NZ_CP066032:3270027-3270278 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References