Detailed information of TA system TA152192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 461453..462048
• Replicon: chromosome
• Accession: NZ_CP050073
• Organism: Enterobacter kobei strain 070

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: HA514_RS02200
• Protein ID: WP_121527375.1
• Coordinates: 461698..462048 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A8B2XPR3
• Locus tag: HA514_RS02195
• Protein ID: WP_032637647.1
• Coordinates: 461453..461704 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA514_RS02185	457120..460896	+	3777	WP_023331875.1	autotransporter assembly complex protein TamB	-
HA514_RS02190	460899..461243	+	345	WP_014882267.1	gamma-glutamylcyclotransferase	-
HA514_RS02195	461453..461704	+	252	WP_032637647.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HA514_RS02200	461698..462048	+	351	WP_121527375.1	type II toxin-antitoxin system ChpB family toxin	Toxin
HA514_RS02205	462126..462656	-	531	WP_008501423.1	inorganic diphosphatase	-
HA514_RS02210	462966..463922	+	957	WP_014882271.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HA514_RS02215	464024..465526	+	1503	WP_045134407.1	sugar ABC transporter ATP-binding protein	-
HA514_RS02220	465537..466562	+	1026	WP_014882273.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12398.34 Da
• Isoelectric Point: 5.8100

>T152192 WP_121527375.1 NZ_CP050073:461698-462048 [Enterobacter kobei]
MVKRPCFERGDIVLVGFDPANGHEQKGAGRPDLVLSVSAFNQLGMTLVAPVTQGGNFARYAGFSVPLSCEEGNIQGVILV
NQIRMMDLGARLAKRIGVASDETVEDALLRLQAILS

Nucleotide

• Length: 351 bp

>T152192 NZ_CP066032:3270027-3270278 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9032.36 Da
• Isoelectric Point: 4.2517

>AT152192 WP_032637647.1 NZ_CP050073:461453-461704 [Enterobacter kobei]
MRITIKKWGNSAGMVIPGVVMKELGLKPGQSMEAQVTNNQLVLTPVSKQYSLEELLAQCDMSAPEINEQDAWGTSGPVGD
EVW

Nucleotide

• Length: 252 bp

>AT152192 NZ_CP066032:3270275-3270529 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XPR3