Detailed information of TA system TA152189

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 379872..380625
• Replicon: plasmid p35
• Accession: NZ_CP050068
• Organism: Klebsiella aerogenes strain 035

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HA513_RS25280
• Protein ID: WP_135683935.1
• Coordinates: 380248..380625 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HA513_RS25275
• Protein ID: WP_162838704.1
• Coordinates: 379872..380198 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA513_RS25215	374930..375196	+	267	WP_009652486.1	hypothetical protein	-
HA513_RS25220	375209..375520	+	312	WP_009652410.1	hypothetical protein	-
HA513_RS25225	375567..376037	+	471	WP_009652431.1	hypothetical protein	-
HA513_RS25230	376086..376598	+	513	WP_009652395.1	DUF4234 domain-containing protein	-
HA513_RS25235	376681..376902	+	222	WP_009652443.1	hypothetical protein	-
HA513_RS25240	376958..377191	+	234	WP_009652474.1	DUF905 domain-containing protein	-
HA513_RS25245	377295..378113	+	819	WP_009652424.1	DUF945 domain-containing protein	-
HA513_RS25250	378113..378367	+	255	WP_009652519.1	hypothetical protein	-
HA513_RS25255	378396..378857	+	462	WP_045890390.1	antirestriction protein	-
HA513_RS25260	378868..379086	+	219	WP_009652503.1	hypothetical protein	-
HA513_RS25265	379098..379580	+	483	WP_052238324.1	DNA repair protein RadC	-
HA513_RS25270	379594..379815	+	222	WP_009652392.1	DUF987 domain-containing protein	-
HA513_RS25275	379872..380198	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HA513_RS25280	380248..380625	+	378	WP_135683935.1	TA system toxin CbtA family protein	Toxin
HA513_RS25285	380622..381113	+	492	WP_009652438.1	hypothetical protein	-
HA513_RS25290	381143..381346	+	204	WP_009652505.1	DUF957 domain-containing protein	-
HA513_RS25295	381427..382272	+	846	Protein_397	DUF4942 domain-containing protein	-
HA513_RS25300	382496..383503	+	1008	Protein_398	hypothetical protein	-
HA513_RS25305	383506..383979	+	474	WP_009652494.1	ribbon-helix-helix protein, CopG family	-
HA513_RS25310	384299..384937	+	639	WP_009652437.1	VWA domain-containing protein	-
HA513_RS25315	384962..385603	+	642	WP_009652489.1	TerD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..397663	397663

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14153.22 Da
• Isoelectric Point: 9.5224

>T152189 WP_135683935.1 NZ_CP050068:380248-380625 [Klebsiella aerogenes]
MQTQPVPPKREVSPRPSPVAIWLRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T152189 NZ_CP066032:2193583-2193990 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTTGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT152189 WP_162838704.1 NZ_CP050068:379872-380198 [Klebsiella aerogenes]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT152189 NZ_CP066032:2193336-2193602 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA