Detailed information of TA system TA152188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 301236..302146
• Replicon: plasmid p35
• Accession: NZ_CP050068
• Organism: Klebsiella aerogenes strain 035

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HA513_RS24830
• Protein ID: WP_004883378.1
• Coordinates: 301236..301706 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HA513_RS24835
• Protein ID: WP_004026357.1
• Coordinates: 301703..302146 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA513_RS24805	297304..297600	-	297	WP_094284716.1	hydrogenase expression/formation protein HypD	-
HA513_RS24810	297831..298208	+	378	WP_004883386.1	hypothetical protein	-
HA513_RS26335	298406..299380	-	975	WP_004883382.1	hypothetical protein	-
HA513_RS24820	299986..300144	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
HA513_RS24825	300867..301127	+	261	WP_004026352.1	hypothetical protein	-
HA513_RS24830	301236..301706	-	471	WP_004883378.1	RES family NAD+ phosphorylase	Toxin
HA513_RS24835	301703..302146	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HA513_RS24840	302246..303517	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
HA513_RS24845	303517..303939	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HA513_RS24850	304294..304863	-	570	Protein_307	transposase	-
HA513_RS24855	304865..305080	+	216	Protein_308	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..397663	397663
	-	flank	IS/Tn	-	-	304294..304680	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17500.82 Da
• Isoelectric Point: 4.6155

>T152188 WP_004883378.1 NZ_CP050068:c301706-301236 [Klebsiella aerogenes]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVLKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T152188 NZ_CP066032:1967575-1967952 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT152188 WP_004026357.1 NZ_CP050068:c302146-301703 [Klebsiella aerogenes]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT152188 NZ_CP066032:1967117-1967485 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V3Q9