Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3766664..3767331 Replicon chromosome
Accession NZ_CP050067
Organism Klebsiella aerogenes strain 035

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3MRP6
Locus tag HA513_RS18365 Protein ID WP_001581456.1
Coordinates 3767002..3767331 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag HA513_RS18360 Protein ID WP_000070396.1
Coordinates 3766664..3766981 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA513_RS18330 3763670..3764367 - 698 Protein_3574 IS1 family transposase -
HA513_RS18335 3764584..3765051 + 468 WP_001581459.1 hypothetical protein -
HA513_RS18340 3765128..3765367 + 240 WP_000194654.1 DUF905 domain-containing protein -
HA513_RS18345 3765465..3765923 + 459 WP_000211838.1 antirestriction protein -
HA513_RS18350 3765939..3766415 + 477 WP_001581457.1 RadC family protein -
HA513_RS18355 3766424..3766645 + 222 WP_000691994.1 DUF987 domain-containing protein -
HA513_RS18360 3766664..3766981 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
HA513_RS18365 3767002..3767331 + 330 WP_001581456.1 toxin Toxin
HA513_RS18370 3767681..3768538 - 858 WP_001581455.1 DeoR/GlpR family DNA-binding transcription regulator -
HA513_RS18375 3768669..3769619 - 951 WP_001581454.1 D-ribose ABC transporter substrate-binding protein -
HA513_RS18380 3769708..3770790 - 1083 WP_006809988.1 ABC transporter permease -
HA513_RS18385 3770793..3772331 - 1539 WP_001581451.1 sugar ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12456.52 Da        Isoelectric Point: 8.5011

>T152186 WP_001581456.1 NZ_CP050067:3767002-3767331 [Klebsiella aerogenes]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK

Download         Length: 330 bp

>T152186 NZ_CP066032:1540565-1540840 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT152186 WP_000070396.1 NZ_CP050067:3766664-3766981 [Klebsiella aerogenes]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT152186 NZ_CP066032:1540837-1541196 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3MRP6

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References