Detailed information of TA system TA152174

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 877021..877678
• Replicon: chromosome
• Accession: NZ_CP050067
• Organism: Klebsiella aerogenes strain 035

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HA513_RS04390
• Protein ID: WP_166479583.1
• Coordinates: 877268..877678 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A0H3FJK6
• Locus tag: HA513_RS04385
• Protein ID: WP_015369791.1
• Coordinates: 877021..877287 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA513_RS04360	872144..873577	-	1434	WP_058675870.1	6-phospho-beta-glucosidase BglA	-
HA513_RS04365	873691..874425	-	735	WP_015703423.1	MurR/RpiR family transcriptional regulator	-
HA513_RS04370	874476..874787	+	312	WP_015703422.1	N(4)-acetylcytidine aminohydrolase	-
HA513_RS04375	874952..875611	+	660	WP_008806429.1	hemolysin III family protein	-
HA513_RS04380	875814..876797	-	984	WP_032712495.1	tRNA-modifying protein YgfZ	-
HA513_RS04385	877021..877287	+	267	WP_015369791.1	FAD assembly factor SdhE	Antitoxin
HA513_RS04390	877268..877678	+	411	WP_166479583.1	protein YgfX	Toxin
HA513_RS04395	877686..878207	-	522	WP_015369793.1	flavodoxin FldB	-
HA513_RS04400	878308..879204	+	897	WP_047080361.1	site-specific tyrosine recombinase XerD	-
HA513_RS04405	879227..879940	+	714	WP_015369795.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HA513_RS04410	879946..881679	+	1734	WP_032715785.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15900.68 Da
• Isoelectric Point: 10.0200

>T152174 WP_166479583.1 NZ_CP050067:877268-877678 [Klebsiella aerogenes]
VVLWQSDLRISWRSQWFSLLLHGVVAAIVLLMPWPLSYTPIWLLLLSLVVFDCVRSQRRIHACQGEIKLLIDSRLRWQKT
EWDIVGTPWVISNGMLLRLKHAESGRSQHLWVAADSMDAGEWRDLRRLVLQKPAHD

Nucleotide

• Length: 411 bp

>T152174 NZ_CP066019:c2724436-2724146 [Cupriavidus necator]
ATGAGGACGATTGAACGGACGACCCAATTCAAGCGCGATTACAAGCGCGAACTCAAAGGGCCGTTCAAGGCGCTGCTGCA
AAGCCCGGCTGGTGACTGGTTTGCAATCCTGACTGCCTTGGCGAGTGACATGTCGCTGCCGGACAAATTTCGCGACCATG
CGCTTGGCGGCCAGTGGAAGGACTTCCGGGATTGCCATGTTCGGCCAGACCTTTTGCTGATCTATCGGAAGCCAGACCCT
TGGACACTGCAACTTGTCCGGCTTGGGTCGCATAGCGAGCTGGCCATGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10579.09 Da
• Isoelectric Point: 5.6963

>AT152174 WP_015369791.1 NZ_CP050067:877021-877287 [Klebsiella aerogenes]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFIRLLENDDPDLFNWLMNHGKPADAQLQRMINLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT152174 NZ_CP066019:c2724689-2724423 [Cupriavidus necator]
ATGTCCGCTTCGACCTTTGTCCGAGCCCGTATCGACGTTGAAATCAAGGAAGAGGCCGCGATCGTTCTGGAAGCGATGGG
ACTGACGGTTTCAGACGCCGTGCGCATGCTTTTGACGCGCGTGGCACGTGAAAAGGCATTGCCTGTGGAACTGATGACTC
CCAATCCAGCAACCATTGCGTCGCTAAAAGAGGCGCGGGCCGGTGGCCTCCGCAAGTTCGGGTCGGTGAGCGACCTGATG
GCGGATCTCAACGATGAGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FJK6