Detailed information of TA system TA152171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3870388..3870919
• Replicon: chromosome
• Accession: NZ_CP050066
• Organism: Bradyrhizobium symbiodeficiens strain 101S1MB

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: HAV00_RS18070
• Protein ID: WP_166468324.1
• Coordinates: 3870388..3870639 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HAV00_RS18075
• Protein ID: WP_094977259.1
• Coordinates: 3870626..3870919 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAV00_RS18050	3865509..3866612	-	1104	WP_166468320.1	mandelate racemase/muconate lactonizing enzyme family protein	-
HAV00_RS18055	3866861..3867787	+	927	WP_166468321.1	SMP-30/gluconolactonase/LRE family protein	-
HAV00_RS18060	3867947..3868540	+	594	WP_166468322.1	GNAT family N-acetyltransferase	-
HAV00_RS18065	3868558..3870204	+	1647	WP_166468323.1	GMC family oxidoreductase N-terminal domain-containing protein	-
HAV00_RS18070	3870388..3870639	+	252	WP_166468324.1	BrnT family toxin	Toxin
HAV00_RS18075	3870626..3870919	+	294	WP_094977259.1	BrnA antitoxin family protein	Antitoxin
HAV00_RS18080	3872142..3872984	-	843	WP_027529387.1	MoxR family ATPase	-
HAV00_RS18085	3873080..3873325	-	246	WP_018318587.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
HAV00_RS18090	3873449..3874624	+	1176	WP_166468325.1	VWA domain-containing protein	-
HAV00_RS18095	3874758..3875171	+	414	WP_094977261.1	rhodanese-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-76)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9566.12 Da
• Isoelectric Point: 10.5508

>T152171 WP_166468324.1 NZ_CP050066:3870388-3870639 [Bradyrhizobium symbiodeficiens]
MKIVWDEPKRLDKHELDFADLNETFFDNALVIPSHNKSKRWVAIGVSIRGVIVVVFARLGREGVSIISMRPASRSERKLY
AER

Nucleotide

• Length: 252 bp

>T152171 NZ_CP066019:1701321-1701599 [Cupriavidus necator]
ATGATTCAGTCATTCAGATGTTCCGACACCGCTGAACTGTTCGCGGGCCGCTGTGTACCGCGCTTCGGCAATATCCGCGC
CGTGGCCGAACGCAAGCTGCGGCTGCTGCAGAGAGCGTCGACGCTGGAGTTCCTGCGCTCCCCGCCGGGCAATCGCCTGC
AGTTGCTGACCGGCGACCGCAAAGGCCGCTACAGCATCCGCATCAACGAGCAATGGCGAATCTGCTTCATCTGGACCCAC
GACGGCCCCGCGGTCGTTGAGATCATCGACTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10779.42 Da
• Isoelectric Point: 11.3548

>AT152171 WP_094977259.1 NZ_CP050066:3870626-3870919 [Bradyrhizobium symbiodeficiens]
MPNAKKKSGYTKRDLRAVSDTPELTRADFAKARPFSEVFPDLSAAIRKGRGPNKAPTKTLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT152171 NZ_CP066019:1701611-1701946 [Cupriavidus necator]
ATGGTTCGCTTCAAGGACGGCATGCGTCCCATCCACCCGGGCGAAATCCTGCGCGAGGATTTCCTGGTACCGCTCAACAT
GAGCGCCAACGCCCTGGCCCTCGCGCTGCGCGTGACCGCGGCGCGCATCAACGACATCGTCCGCGAGCAACGTGGCATCA
CGCCGGACACCGCCCTGCGCCTGGCGCGCTATTTTGGCGGCGATGCGCAGTCGTGGCTGAACCTGCAGCTGGACTATGAC
CTGAAGGTCGCGCAGCGCGACCACGGCGAGCGCATCATGGCGGAAGTGGCGCCGCGCGATGCCGCGGCGGCCGGTCAACC
GGCCGCTGAAAAGTAG