Detailed information of TA system TA152167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 5733355..5734892
• Replicon: chromosome
• Accession: NZ_CP050064
• Organism: Bradyrhizobium diazoefficiens strain 172S4

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A837C678
• Locus tag: HAU57_RS27145
• Protein ID: WP_049832575.1
• Coordinates: 5733355..5734278 (-)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: HAU57_RS27150
• Protein ID: WP_074077775.1
• Coordinates: 5734275..5734892 (-)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAU57_RS27115	5728423..5729592	+	1170	WP_039187218.1	site-specific integrase	-
HAU57_RS27120	5729627..5730145	+	519	WP_131234233.1	hypothetical protein	-
HAU57_RS27125	5730161..5730874	+	714	WP_131234232.1	hypothetical protein	-
HAU57_RS27130	5730951..5731157	+	207	WP_028175471.1	helix-turn-helix domain-containing protein	-
HAU57_RS27135	5731189..5732115	+	927	WP_157862142.1	toprim domain-containing protein	-
HAU57_RS27140	5732118..5733200	+	1083	WP_049832576.1	AAA family ATPase	-
HAU57_RS27145	5733355..5734278	-	924	WP_049832575.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HAU57_RS27150	5734275..5734892	-	618	WP_074077775.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HAU57_RS27155	5735141..5739199	+	4059	WP_028175467.1	helix-turn-helix domain-containing protein	-
HAU57_RS27160	5739326..5739883	+	558	WP_074077613.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5729627..5750761	21134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-276)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-56)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 34339.39 Da
• Isoelectric Point: 4.9777

>T152167 WP_049832575.1 NZ_CP050064:c5734278-5733355 [Bradyrhizobium diazoefficiens]
MIGKPLKDPGASIRQRLLNHAKEHGDEYQRILTRYAIERLLFRLSQTEARERYVLKGAMLFVTWPEQVFRPTGDLDLLGQ
GDPDPAAIIDLFTLICQVEAPADGIIFDPSTLQVEAVREEEKYQGVRLSLRGRLGTAVIPVQVDIGFGDSVHPAPSRNNF
PSLLPDLPAADVLMYPPETVIAEKFEAMIRFGEANGRIKDFHDIWVTARTFAFNLSTLVEAVSGTLQRRETAFPTEMPVA
LTSQFAEMADKQALWTGFLRRNPPTLPPPPLGDLLAELRRFFEPVLAALPLPESATGRWNPDRGAWE

Nucleotide

• Length: 924 bp

>T152167 NZ_CP066018:4002917-4003216 [Cupriavidus necator]
ATGCTCAGCATCCGGACAACCCAAATTTCCGACCGCTGGTATTCGGCCTTGCGCGACCACGTCGCCAGGATTCGCATCCA
GGTCCGCGTCGACCGCTTACTGAACGGGAATCCCGGCGACGCTAAAGCCCTTGGCGGTGGAGTCAGCGAAATGCGCGTGG
ACCACGGCCCGGGCTACCGCATCTACTTTCAGCAACGCGGCCAGGTAATTGTGATCCTGCTCTGCGGAGGCAACAAGTCG
ACCCAGCACGCTGATATCGAAAGAGCACGCAGTCTGGCGGCCAATCTTGATCTGGAGTGA

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22770.44 Da
• Isoelectric Point: 10.4747

>AT152167 WP_074077775.1 NZ_CP050064:c5734892-5734275 [Bradyrhizobium diazoefficiens]
MTQRHVLPYRERALNLVRARGIARARDFKAAGIPLVYLKRLTDTGELVRLGRGLYQDPDRVGEDVAHNLAEAARAVPNGV
VSLASALRYHGLTTQLPHAVWLTIPHKARTPKAIGFPLEIVRATGDVMTAGVEHVQVEGVDVPIYGVAKTIADCFKHRRH
IGEDVAIEALRDALRLRKTTVSDVMRYAEIDRVAERIRPVLKALQ

Nucleotide

• Length: 618 bp

>AT152167 NZ_CP066018:4003220-4003510 [Cupriavidus necator]
ATGAAGGAACCAACCCGCCCCTGGGACTCCGCCGAGCATCTGAGGAACGATCAGGAGATCGCCGCCTACCTGGATGCCTG
CCTGGCCGAGGCCGGCGACGACGATCGATTCATTGCCTACGCACTCGGGGTGGTGGCGCGTGCGCGCGGCATGACGCGAC
TGGCCCGCGAAACGGGCCTTTCGCGCGAAAGCCTCTACCGCTCGCTCTCAGGCGAAGGCAATCCGGAGTTCGGGACGATC
TGGAAGGTGATGCGGGCGCTGGGCATCCGCCTGCATGCGAGCGCTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A837C678