Detailed information of TA system TA152146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2019..2554
• Replicon: plasmid pCD90-1-1
• Accession: NZ_CP050048
• Organism: Escherichia coli strain 90-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G9P52_RS22975
• Protein ID: WP_038976993.1
• Coordinates: 2267..2554 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: G9P52_RS22970
• Protein ID: WP_001139206.1
• Coordinates: 2019..2270 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P52_RS22965	46..1077	+	1032	WP_080182593.1	replication initiation protein	-
G9P52_RS22970	2019..2270	+	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G9P52_RS22975	2267..2554	+	288	WP_038976993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G9P52_RS22980	2842..3045	-	204	WP_038976994.1	alkaline phosphatase	-
G9P52_RS22985	3035..3240	+	206	Protein_4	transposase	-
G9P52_RS22990	3416..6913	-	3498	WP_113078458.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105583	105583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11113.16 Da
• Isoelectric Point: 10.6478

>T152146 WP_038976993.1 NZ_CP050048:2267-2554 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDKLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T152146 NZ_CP065999:1686519-1686818 [Kingella denitrificans]
GTGGAAAAGCGTACGCCGCATTGCAAATTGGAAATCGTGAAAGCATTGATTGCGGAAGGACGCGTTGCGGCAACGTTTTC
TGCAAGGCGTACCGCACAAGAAGCGGGAATTGATTATGCCGCGATGGTGGAAATCGTAAATAACTTGAACCGTTCGGATT
TCTACAAAAGCATGACGACCCATGCCGATCATACCGTTTGGCAGGATGTATACCGCCCGAACGTTGAGCCTTACGGCGGT
TTATATATCAAGTTGACTGTGATTGATGATGTGTTAATTGTTTCATTTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT152146 WP_001139206.1 NZ_CP050048:2019-2270 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT152146 NZ_CP065999:1686820-1687224 [Kingella denitrificans]
ATGAAATGTCCTGTGTGCGGCGCAGCCGAGTTGGTGCACGATGTGCGCGATGTCCCTTATACCTATAAGGGGCAAACAAC
CGTCCTGCCCCATGTGGAAGCGGATTATTGCGATGCCTGCGGAGACCATCTGACCGGACCTGCGGAGGGCAAGCGGACAA
TGGATTTAATGGGTGCGTTTATCAAACAGGTAAACGAGCGGCAAGCCGACCCCAATTTCATCCGCAGCGTCCGTGCCAAA
CTGAACCTGACCCAGCGCGAAGCGGGGGATTTGTTCGGCGGCGGCGTGAATGCTTTTTCACGTTATGAGAGCGGGAAGGT
CGAGCCGCCAAAATCCCTGTCTATCCTGTTTGCTTTGTTGGATGAAAATCCTGCTTTGCTGCAAAGGATAAGGCAGGCAG
GCTGA

Structures

Antitoxin

• AlphaFold DB: T1PXV2