Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4361856..4362481 Replicon chromosome
Accession NZ_CP050047
Organism Escherichia coli strain 90-1

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag G9P52_RS20915 Protein ID WP_000911329.1
Coordinates 4362083..4362481 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag G9P52_RS20910 Protein ID WP_000450524.1
Coordinates 4361856..4362083 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P52_RS20885 4357658..4358128 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
G9P52_RS20890 4358128..4358700 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
G9P52_RS20895 4358846..4359724 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
G9P52_RS20900 4359741..4360775 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
G9P52_RS20905 4360988..4361701 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
G9P52_RS20910 4361856..4362083 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
G9P52_RS20915 4362083..4362481 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
G9P52_RS20920 4362628..4363491 + 864 WP_001267498.1 neutral zinc metallopeptidase -
G9P52_RS20925 4363506..4365521 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
G9P52_RS20930 4365595..4366293 + 699 WP_000679812.1 esterase -
G9P52_RS20935 4366403..4366603 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T152145 WP_000911329.1 NZ_CP050047:4362083-4362481 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T152145 NZ_CP065998:c2097413-2097147 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTCTTAATTAAGAATTCAGCCAAAAATGACTTGAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCATATTATGAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT152145 WP_000450524.1 NZ_CP050047:4361856-4362083 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT152145 NZ_CP065998:c2097670-2097413 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATGGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References