Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3908893..3909547 Replicon chromosome
Accession NZ_CP050047
Organism Escherichia coli strain 90-1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag G9P52_RS18795 Protein ID WP_000244772.1
Coordinates 3909140..3909547 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G9P52_RS18790 Protein ID WP_000354046.1
Coordinates 3908893..3909159 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P52_RS18765 3904181..3904924 + 744 WP_001436959.1 SDR family oxidoreductase -
G9P52_RS18770 3904981..3906414 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
G9P52_RS18775 3906459..3906770 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G9P52_RS18780 3906934..3907593 + 660 WP_000250274.1 hemolysin III family protein -
G9P52_RS18785 3907670..3908650 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G9P52_RS18790 3908893..3909159 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G9P52_RS18795 3909140..3909547 + 408 WP_000244772.1 protein YgfX Toxin
G9P52_RS18800 3909587..3910108 - 522 WP_001055874.1 flavodoxin FldB -
G9P52_RS18805 3910220..3911116 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G9P52_RS18810 3911141..3911851 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G9P52_RS18815 3911857..3913590 + 1734 WP_000813213.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T152143 WP_000244772.1 NZ_CP050047:3909140-3909547 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T152143 NZ_CP065998:139518-139739 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT152143 WP_000354046.1 NZ_CP050047:3908893-3909159 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT152143 NZ_CP065998:139218-139505 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References