Detailed information of TA system TA152141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3361421..3362221
• Replicon: chromosome
• Accession: NZ_CP050047
• Organism: Escherichia coli strain 90-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: G9P52_RS16055
• Protein ID: WP_000342449.1
• Coordinates: 3361694..3362221 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: G9P52_RS16050
• Protein ID: WP_160438549.1
• Coordinates: 3361421..3361687 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P52_RS16030	3357079..3357747	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
G9P52_RS16035	3357740..3358798	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
G9P52_RS16040	3359043..3359897	+	855	WP_000130221.1	RNA polymerase sigma factor RpoH	-
G9P52_RS16045	3360168..3361271	+	1104	WP_001350438.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
G9P52_RS16050	3361421..3361687	+	267	WP_160438549.1	DUF1778 domain-containing protein	Antitoxin
G9P52_RS16055	3361694..3362221	+	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
G9P52_RS16060	3362218..3362601	-	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
G9P52_RS16065	3363025..3364134	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
G9P52_RS16070	3364182..3365108	+	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
G9P52_RS16075	3365105..3366382	+	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
G9P52_RS16080	3366379..3367146	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T152141 WP_000342449.1 NZ_CP050047:3361694-3362221 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T152141 NZ_CP065997:6020025-6020339 [Achromobacter deleyi]
ATGTACCACATAAAGCCGCTGCCAGAGTTCGACGCTTGGTTCGACACCCTGGCAGACCCGGCTACGCGAGCCCGCCTGCT
CCGCAGGCTTGAAAAGGCCGCGCGGGGTTTGCTGGGCGATGTGAAACCGGTCGGAGAGGGCGTGAGCGAGATGCGCGAGT
TCTTTGGCCCTGGCTGGCGCACGTATTACACCGAGCAGGGCGACGTGCTGATCGTCATGCTGGGCGGCGGCACACAGGCC
ACACAAAGCCGTGACGTCAAGCACGCCAAGGAATTGGCGCGGGCTCTGAGAAACGACGAGGAAGACCAATCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9944.33 Da
• Isoelectric Point: 10.0863

>AT152141 WP_160438549.1 NZ_CP050047:3361421-3361687 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRFQGM

Nucleotide

• Length: 267 bp

>AT152141 NZ_CP065997:6020336-6020647 [Achromobacter deleyi]
ATGAGCAAGAAGACGATCAAGGTTTCCGAACTGCCCACCTTCGACCCTGCGCGTTACCTGCGCGACGACAAGGACATCGC
CGCCTACTTGACGCAGGTGATCGAGGACGGAGACTTCGGTGAGCTTGCCCAGGCGCTGGGAGTTGCCGCGCGCGCTCGCG
GCATGACGCAAATTGCCGAGACTGCCGGTATTGGCCGGGAATCCCTGTATAAGGCCCTGCGGGCTGGCGCCAGCCCGCGC
TTTGACACCATCGCCAAGGTATGCAAGGCACTGGGCGTACGCCTTGTCGTCCAGGCCGAACATCCGGCCTAG

Structures

Toxin

• AlphaFold DB: A0A829CLZ4