Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2550862..2551359 Replicon chromosome
Accession NZ_CP050047
Organism Escherichia coli strain 90-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G9P52_RS12370 Protein ID WP_001173343.1
Coordinates 2550862..2551035 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G9P52_RS12375 Protein ID WP_000398619.1
Coordinates 2551063..2551359 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P52_RS12360 2547532..2548989 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
G9P52_RS12365 2549226..2550743 + 1518 WP_001295087.1 lysine--tRNA ligase -
G9P52_RS12370 2550862..2551035 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G9P52_RS12375 2551063..2551359 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G9P52_RS12380 2551586..2551858 - 273 WP_000405647.1 N-acetyltransferase -
G9P52_RS12385 2551870..2552100 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
G9P52_RS12390 2552281..2553912 + 1632 WP_001216487.1 sensor histidine kinase -
G9P52_RS12395 2553909..2554628 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T152137 WP_001173343.1 NZ_CP050047:c2551035-2550862 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T152137 NZ_CP065989:c3357513-3357223 [Brevibacterium casei]
GTGAATCGCTATCGCCTCTCAGCTGCGGCTCAGCGAGACCTCTCCGAGATCTGGGATTACACCGAACGGCGCTGGGACAG
CAATCAGGCCGAGAACTACATGCAGCAGATCAGATCAGCAGTCGAGCACATCGCTGCCGACCCTGGTAGAGGTCGCGCCG
TGAACGAGATTCGAGAGGGCTATCGCAGATTCGCGACCGGGAGCCATGTCCTCTTCTACCGGGTCCGACCCAAGGGGGTG
GACGTCATCAGAGTGCTCCATCAGCGCATGGATCCCACTCGCCACCTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT152137 WP_000398619.1 NZ_CP050047:c2551359-2551063 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT152137 NZ_CP065989:c3357743-3357510 [Brevibacterium casei]
ATGGCTCAGAACACATCGATCAGCTTGGACGATCATTTCGTGGATTTCCTGTCTCAAGAAGTCGCCTCGGGTCGATACCA
GTCCGCGAGTGAGGTCGTGCGGGCGGGACTGCGCATGCTGGAGGATCACGAAACCCAGATGGCCACTCTGCGGGCGGCAT
TGATCGCGGGAGAGGAGAGCGGTGAACCTGAGGACTTCGACTTTGAGGAGTTCATCGCCGCTAAGCGGTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References