Detailed information of TA system TA152135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2393703..2395386
• Replicon: chromosome
• Accession: NZ_CP050047
• Organism: Escherichia coli strain 90-1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: G9P52_RS11600
• Protein ID: WP_000361918.1
• Coordinates: 2394469..2395386 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: G9P52_RS11595
• Protein ID: WP_000157137.1
• Coordinates: 2393703..2394476 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P52_RS11580	2389571..2391655	+	2085	WP_001462982.1	protease Lon-related BREX system protein BrxL	-
G9P52_RS11585	2391774..2393123	+	1350	WP_188028011.1	phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase family protein	-
G9P52_RS11590	2393299..2393619	-	321	Protein_2270	IS3 family transposase	-
G9P52_RS11595	2393703..2394476	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
G9P52_RS11600	2394469..2395386	+	918	WP_000361918.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G9P52_RS11605	2395680..2395847	+	168	WP_001356018.1	hypothetical protein	-
G9P52_RS11610	2395900..2396562	-	663	WP_000357928.1	hypothetical protein	-
G9P52_RS11615	2396566..2397276	-	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
G9P52_RS11620	2397992..2398456	-	465	WP_000749342.1	helix-turn-helix domain-containing protein	-
G9P52_RS11625	2398696..2399193	+	498	WP_000148193.1	hypothetical protein	-
G9P52_RS11630	2399244..2400092	-	849	WP_007866156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2384065..2402660	18595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34962.10 Da
• Isoelectric Point: 4.9206

>T152135 WP_000361918.1 NZ_CP050047:2394469-2395386 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T152135 NZ_CP065983:c1432232-1430952 [Aggregatibacter segnis]
ATGAGTAACTCGATTCTAAACGTCGCCATGAATGGCTTGTTAGTCGGTGAATGGCGAAAACTCACCAGTGGCGCAACCGA
ATTTCAGTATGCGGAAAAATGGCTTGAATCTGTTCGCTCGCGCCCTATTTCATTGTCACTGCCCTTGTCGCACAAAATTT
ATCGAGGAGATTCCGTTTACAATTTCTTCGATAATCTCCTCCCGGACAATGAGCAGATCCGTTCACGCATTCAGCAACGT
TTTCAAACCTCAACAAAATCCCCATTTGATTTACTTTCGGCGATTGGACAGGATTGTATCGGCGCAATTCAGCTTTATCA
GGGTGAGAACCGGTCGGTACGTCAAATTTATGCAGAACCGTTAAATGACAAAGAGGTGGAAAACGTGCTGAAAAACTACC
GCACTTTTCCTCTTGGTATGGAAGAAAGCGAGGATTTCCGAATTTCTTTAGCCGGCGCTCAGGAAAAAACAGCGCTGCTT
TATTATCAAAATCAATGGCAGCGCCCTTTGCATTCCACCGCGACCAGCCATATTTTTAAACTCCCGATTGGTATGGTGGC
ACAAGGGCAACTTGATCTTTCCGGTAGCTGTGAAAATGAATGGCTTTGCTTGAATATTATGAAAGCCTTCGGTTTGCCGG
TTCCTCGAAGCCAAATTCAGTATTTCGGTGAAATGAAAGTGCTGATTGTGGAGCGCTTCGATCGCCGTTGGTCTGAAAAT
AGAAGTTGGCTAATTCGTTTACCACAGGAGGATATGTGCCAAGCACTCAACATTGCCCCTGCGCGAAAATATGAAAATGA
CGGCGGGCCGAGCATTCTTCAAATTATGTCGCTTCTCAAGGGTTCATCCAACGCCCAAGCAGATCGCAAGCAGTTTTTTA
AAGCGCAAATTATTTTCTGGCTGCTTTGTGCCATTGACGGCCATGGCAAAAATTTCAGTCTATTTTTGGAACAAGAGAAT
CGCTATCGTCTAACGCCTTTTTATGACGTGATGTCCGCCTATCCGCTCATCAACCACAACGGACTACAAAAACAAAAAGT
CAAAATGGCAATGGCGTGGCACGGCAAAAATAAACATTATTTATGGGATAAAATACAACCCCGCCACATTTTCAACACCG
CTAAACTCGCAGGCTTGTCAAAGGAAACAGTTGAAGACGTTTTGCATGAGATCAGTGCGCTATATCGAAGAGTCAGTGAA
ATAAATACCCATGGATTGCCTGATGAAATTGTGGAGCCAATTTTAATGGGATTGGAAATGCAGATGAAAAAGATAAGTTA
A

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT152135 WP_000157137.1 NZ_CP050047:2393703-2394476 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT152135 NZ_CP065983:c1432510-1432232 [Aggregatibacter segnis]
ATGGTTATCACCACCGCAAAAATGCTTTCTCATGTAATTCGGGAATTTCGCTATCAAGGCGATCTGTCACAAACTGATGT
TGCTAAACTCGCCGGAACGACCCAAGCCCGAATTTCAGCGTTTGAGAATGAGCCGGAGCGTACTAAATTAGAAACGCTAT
TTAAAATTCTGGCAGGACTAGAACTAGAGCTTATTGTGCAACCACGCAAAAAGTCATCTAATACCGCTCTCTCAATTAAG
GAGCCAACAGCAACATACGATGACGATGAGGCATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2