152133

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1796769..1797606 Replicon chromosome
Accession NZ_CP050047
Organism Escherichia coli strain 90-1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag G9P52_RS08760 Protein ID WP_000227784.1
Coordinates 1797064..1797606 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag G9P52_RS08755 Protein ID WP_001297137.1
Coordinates 1796769..1797080 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P52_RS08730 1791789..1792736 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
G9P52_RS08735 1792758..1794749 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
G9P52_RS08740 1794739..1795353 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
G9P52_RS08745 1795353..1795682 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
G9P52_RS08750 1795694..1796584 + 891 WP_000971336.1 heme o synthase -
G9P52_RS08755 1796769..1797080 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
G9P52_RS08760 1797064..1797606 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
G9P52_RS08765 1797662..1798597 - 936 WP_001297127.1 sel1 repeat family protein -
G9P52_RS08770 1799005..1800369 + 1365 WP_001000978.1 MFS transporter -
G9P52_RS08775 1800497..1800988 - 492 WP_001138904.1 nucleotide binding protein YajQ -
G9P52_RS08780 1801156..1802067 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T152133 WP_000227784.1 NZ_CP050047:1797064-1797606 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T152133 NZ_CP065981:c366504-366232 [Macrococcus caseolyticus]
ATGCCTAAGAAAAAGATACTATTTTATTCAAGTGGTTTTGAAGATTATACGTACTGGCAATCTAACGATAAAACTGTATT
AAAGAGAATAAATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGAAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCCATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT152133 WP_001297137.1 NZ_CP050047:1796769-1797080 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT152133 NZ_CP065981:c366754-366497 [Macrococcus caseolyticus]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGTATAATGCCGTGATATTATCTGAACAAGATTATAATAGTATGATTGAAACACTTTATTTAC
AGAGTACACCAGCGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References