Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 37616..38138 Replicon plasmid pCD74-2-2
Accession NZ_CP050046
Organism Escherichia coli strain 74-2

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag G9P51_RS23430 Protein ID WP_000220560.1
Coordinates 37857..38138 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag G9P51_RS23425 Protein ID WP_000121743.1
Coordinates 37616..37867 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P51_RS23400 33287..33568 + 282 WP_001185482.1 hypothetical protein -
G9P51_RS23405 33593..34570 + 978 WP_024245156.1 hypothetical protein -
G9P51_RS23410 34567..34992 - 426 WP_000202942.1 hypothetical protein -
G9P51_RS23415 35060..35506 - 447 Protein_39 hypothetical protein -
G9P51_RS23420 35546..36382 - 837 WP_001050931.1 replication initiation protein -
G9P51_RS23425 37616..37867 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
G9P51_RS23430 37857..38138 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G9P51_RS23435 38213..38910 + 698 Protein_43 IS1 family transposase -
G9P51_RS23440 38920..39228 + 309 Protein_44 replication initiation protein -
G9P51_RS23445 40076..41044 + 969 WP_030005799.1 IS5 family transposase -
G9P51_RS23450 41184..41570 - 387 WP_001039464.1 hypothetical protein -
G9P51_RS23455 42151..43089 + 939 WP_077817178.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / blaTEM-1B / sul3 / tet(A) / floR / tet(X4) / dfrA14 / aph(6)-Id - 1..44690 44690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T152120 WP_000220560.1 NZ_CP050046:37857-38138 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T152120 NZ_CP065970:c2867034-2866660 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT152120 WP_000121743.1 NZ_CP050046:37616-37867 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT152120 NZ_CP065970:c2867491-2867123 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References