Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 23067..23668 Replicon plasmid pCD74-2-1
Accession NZ_CP050045
Organism Escherichia coli strain 74-2

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag G9P51_RS22760 Protein ID WP_001216039.1
Coordinates 23067..23447 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID -
Locus tag G9P51_RS22765 Protein ID WP_001190711.1
Coordinates 23447..23668 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P51_RS22725 18129..19160 - 1032 WP_000067537.1 site-specific integrase -
G9P51_RS22730 19168..19389 - 222 WP_000542338.1 hypothetical protein -
G9P51_RS22735 19994..20203 + 210 WP_000874156.1 hypothetical protein -
G9P51_RS22740 20314..21165 + 852 WP_000611661.1 hypothetical protein -
G9P51_RS22745 21198..21509 - 312 WP_001351993.1 phage antirepressor Ant -
G9P51_RS22750 21812..22855 - 1044 WP_069067567.1 DUF968 domain-containing protein -
G9P51_RS22755 22883..23062 - 180 WP_000113018.1 hypothetical protein -
G9P51_RS22760 23067..23447 - 381 WP_001216039.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
G9P51_RS22765 23447..23668 - 222 WP_001190711.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
G9P51_RS22770 23933..24630 + 698 Protein_29 IS1 family transposase -
G9P51_RS22775 24647..24973 - 327 Protein_30 hypothetical protein -
G9P51_RS22780 25046..26308 - 1263 WP_069067570.1 hypothetical protein -
G9P51_RS22785 26610..27311 - 702 WP_000684873.1 hypothetical protein -
G9P51_RS22790 27308..27856 - 549 Protein_33 metallophosphoesterase -
G9P51_RS22795 27872..28569 - 698 Protein_34 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..112291 112291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13558.27 Da        Isoelectric Point: 5.1514

>T152119 WP_001216039.1 NZ_CP050045:c23447-23067 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T152119 NZ_CP065970:2855138-2855392 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8134.08 Da        Isoelectric Point: 4.5843

>AT152119 WP_001190711.1 NZ_CP050045:c23668-23447 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Download         Length: 222 bp

>AT152119 NZ_CP065970:2854890-2855141 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References