Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2488646..2489284 Replicon chromosome
Accession NZ_CP050044
Organism Escherichia coli strain 74-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G9P51_RS11805 Protein ID WP_000813794.1
Coordinates 2489108..2489284 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G9P51_RS11800 Protein ID WP_001270286.1
Coordinates 2488646..2489062 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P51_RS11780 2483798..2484739 - 942 WP_001251313.1 ABC transporter permease -
G9P51_RS11785 2484740..2485753 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
G9P51_RS11790 2485771..2486916 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
G9P51_RS11795 2487161..2488567 - 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
G9P51_RS11800 2488646..2489062 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G9P51_RS11805 2489108..2489284 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G9P51_RS11810 2489506..2489736 + 231 WP_000494244.1 YncJ family protein -
G9P51_RS11815 2489828..2491789 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G9P51_RS11820 2491862..2492398 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
G9P51_RS11825 2492451..2493665 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2493705..2494970 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T152114 WP_000813794.1 NZ_CP050044:c2489284-2489108 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T152114 NZ_CP065970:1927922-1928329 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT152114 WP_001270286.1 NZ_CP050044:c2489062-2488646 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT152114 NZ_CP065970:1927675-1927941 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References