Detailed information of TA system TA152100

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 728297..729132
• Replicon: chromosome
• Accession: NZ_CP050044
• Organism: Escherichia coli strain 74-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A763DSF8
• Locus tag: G9P51_RS03435
• Protein ID: WP_047928871.1
• Coordinates: 728755..729132 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G9P51_RS03430
• Protein ID: WP_047928872.1
• Coordinates: 728297..728665 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P51_RS03395	724845..725078	+	234	WP_047928875.1	DUF905 family protein	-
G9P51_RS03400	725169..725987	+	819	WP_096935434.1	DUF945 domain-containing protein	-
G9P51_RS03405	725987..726232	+	246	WP_069723089.1	antirestriction protein	-
G9P51_RS03410	726326..726799	+	474	WP_112026839.1	antirestriction protein	-
G9P51_RS03415	726815..727291	+	477	WP_001186770.1	RadC family protein	-
G9P51_RS03420	727360..727581	+	222	WP_000692312.1	DUF987 domain-containing protein	-
G9P51_RS03425	727600..728247	+	648	WP_114493062.1	antitoxin of toxin-antitoxin stability system	-
G9P51_RS03430	728297..728665	+	369	WP_047928872.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G9P51_RS03435	728755..729132	+	378	WP_047928871.1	TA system toxin CbtA family protein	Toxin
G9P51_RS03440	729129..729278	+	150	Protein_674	hypothetical protein	-
G9P51_RS03445	729354..729551	+	198	WP_096093965.1	DUF957 domain-containing protein	-
G9P51_RS03450	729636..730481	+	846	WP_072650597.1	DUF4942 domain-containing protein	-
G9P51_RS03460	730781..731287	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
G9P51_RS03465	731366..733207	-	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13941.88 Da
• Isoelectric Point: 7.3222

>T152100 WP_047928871.1 NZ_CP050044:728755-729132 [Escherichia coli]
MKTLPVLPGQAAGSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNGITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T152100 NZ_CP065966:c2286931-2286641 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13757.47 Da
• Isoelectric Point: 5.0907

>AT152100 WP_047928872.1 NZ_CP050044:728297-728665 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEDNQLHYLADRAGIRGRFSDADAYHLDQALPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT152100 NZ_CP065966:c2287227-2286928 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Toxin

• AlphaFold DB: A0A763DSF8