152093

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3842804..3843380 Replicon chromosome
Accession NZ_CP050040
Organism Escherichia coli strain 63-2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag G9P50_RS18315 Protein ID WP_001297643.1
Coordinates 3843093..3843380 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag G9P50_RS18310 Protein ID WP_000063156.1
Coordinates 3842804..3843106 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P50_RS18295 3839400..3841550 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
G9P50_RS18300 3841600..3841803 + 204 WP_000467859.1 YbdD/YjiX family protein -
G9P50_RS18305 3841814..3842770 + 957 WP_001297640.1 GTPase -
G9P50_RS18310 3842804..3843106 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
G9P50_RS18315 3843093..3843380 - 288 WP_001297643.1 BrnT family toxin Toxin
G9P50_RS18320 3843579..3844276 - 698 Protein_3576 IS1 family transposase -
G9P50_RS18325 3844333..3844428 + 96 Protein_3577 dienelactone hydrolase family protein -
G9P50_RS18330 3844574..3845806 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
G9P50_RS18335 3845847..3847127 + 1281 WP_032292030.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T152093 WP_001297643.1 NZ_CP050040:c3843380-3843093 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T152093 NZ_CP065964:c1326709-1326428 [Corynebacterium minutissimum]
ATGATTCAGTCGTTTGCTAACCGAGACACCGAGCTTGTCTGGTTGCGCCAAGCGTCACCCCGGATCGATTCAAGAATTCA
CAAGACTGCAAATAGAAAACTTCATCAGCTTGATGCAGCAGTTTCCCTGCAATCGCTGCGTGTTCCGCCGGGGAACCGAT
TGGAAGCTTTGAAAGGTGATCGAAAGGGGATGTACAGCATTCGAGTCAATCAACAATGGAGGATTACTTTTCGCTGGACC
GAAGCGGGCCCGGCGGATGTGGCAATAGAGGATTATCACTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT152093 WP_000063156.1 NZ_CP050040:c3843106-3842804 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT152093 NZ_CP065964:c1326419-1326120 [Corynebacterium minutissimum]
ATGAGTACCAAGTTGTATCCACCTATTCATCCTGGAGAAGTACTCAACGAAGACTTCATTAAGGCATTTGGGATTACCCA
GCATAAGTTAGCTATGGCCATTGGAGTCCCTCCGCGCAGGATTAACGAGATCGTCCACGGCAAGCGCGGAATTACAGCAG
ACACTGCGCTGCGTTTAGGAAGGTATTTCGGGGTAGAACCGCAGTTCTGGGTGAACTTGCAAGGACGATACGAGCTCGAA
ATAGCAGAGGATTCAATCGCTGAAGAAGTCAGCAGTATTAAGCCGCTCGAAGTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References