Detailed information of TA system TA152090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2344561..2345199
• Replicon: chromosome
• Accession: NZ_CP050040
• Organism: Escherichia coli strain 63-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: G9P50_RS11145
• Protein ID: WP_000813794.1
• Coordinates: 2345023..2345199 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G9P50_RS11140
• Protein ID: WP_001270286.1
• Coordinates: 2344561..2344977 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P50_RS11120	2339713..2340654	-	942	WP_001251313.1	ABC transporter permease	-
G9P50_RS11125	2340655..2341668	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
G9P50_RS11130	2341686..2342831	-	1146	WP_182143872.1	ABC transporter substrate-binding protein	-
G9P50_RS11135	2343076..2344482	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
G9P50_RS11140	2344561..2344977	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G9P50_RS11145	2345023..2345199	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G9P50_RS11150	2345421..2345651	+	231	WP_000494244.1	YncJ family protein	-
G9P50_RS11155	2345743..2347704	-	1962	WP_001752477.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G9P50_RS11160	2347777..2348313	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
G9P50_RS11165	2348366..2349595	+	1230	WP_071531711.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2349635..2350783	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T152090 WP_000813794.1 NZ_CP050040:c2345199-2345023 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T152090 NZ_CP065953:234931-235215 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT152090 WP_001270286.1 NZ_CP050040:c2344977-2344561 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT152090 NZ_CP065953:234693-234941 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Structures

Toxin

• PDB: 6HPB