Detailed information of TA system TA152078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1258745..1259370
• Replicon: chromosome
• Accession: NZ_CP050040
• Organism: Escherichia coli strain 63-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G9P50_RS06030
• Protein ID: WP_000911330.1
• Coordinates: 1258972..1259370 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: G9P50_RS06025
• Protein ID: WP_000450524.1
• Coordinates: 1258745..1258972 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P50_RS06000	1254547..1255017	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
G9P50_RS06005	1255017..1255589	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
G9P50_RS06010	1255735..1256613	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
G9P50_RS06015	1256630..1257664	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
G9P50_RS06020	1257877..1258590	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
G9P50_RS06025	1258745..1258972	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G9P50_RS06030	1258972..1259370	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G9P50_RS06035	1259517..1260380	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
G9P50_RS06040	1260395..1262410	+	2016	WP_000829336.1	tRNA cytosine(34) acetyltransferase TmcA	-
G9P50_RS06045	1262484..1263182	+	699	WP_000679812.1	esterase	-
G9P50_RS06050	1263292..1263492	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T152078 WP_000911330.1 NZ_CP050040:1258972-1259370 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T152078 NZ_CP065949:890089-890496 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT152078 WP_000450524.1 NZ_CP050040:1258745-1258972 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT152078 NZ_CP065949:889842-890108 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33