Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2451660..2452298 Replicon chromosome
Accession NZ_CP050036
Organism Escherichia coli strain 58-3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G9P49_RS11880 Protein ID WP_000813794.1
Coordinates 2452122..2452298 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G9P49_RS11875 Protein ID WP_001270286.1
Coordinates 2451660..2452076 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P49_RS11855 2446812..2447753 - 942 WP_001251313.1 ABC transporter permease -
G9P49_RS11860 2447754..2448767 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
G9P49_RS11865 2448785..2449930 - 1146 WP_182116710.1 ABC transporter substrate-binding protein -
G9P49_RS11870 2450175..2451581 - 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
G9P49_RS11875 2451660..2452076 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G9P49_RS11880 2452122..2452298 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G9P49_RS11885 2452520..2452750 + 231 WP_000494244.1 YncJ family protein -
G9P49_RS11890 2452842..2454803 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G9P49_RS11895 2454876..2455412 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
G9P49_RS11900 2455498..2456679 + 1182 WP_182116711.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T152067 WP_000813794.1 NZ_CP050036:c2452298-2452122 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T152067 NZ_CP065947:3107482-3107760 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT152067 WP_001270286.1 NZ_CP050036:c2452076-2451660 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT152067 NZ_CP065947:3107772-3108077 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References