Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 975421..976004 Replicon chromosome
Accession NZ_CP050036
Organism Escherichia coli strain 58-3

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag G9P49_RS04705 Protein ID WP_000254738.1
Coordinates 975669..976004 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag G9P49_RS04700 Protein ID WP_000581941.1
Coordinates 975421..975669 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P49_RS04690 971760..973061 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
G9P49_RS04695 973109..975343 + 2235 WP_000226815.1 GTP pyrophosphokinase -
G9P49_RS04700 975421..975669 + 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
G9P49_RS04705 975669..976004 + 336 WP_000254738.1 endoribonuclease MazF Toxin
G9P49_RS04710 976075..976866 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
G9P49_RS04715 977094..978731 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
G9P49_RS04720 978819..980117 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T152055 WP_000254738.1 NZ_CP050036:975669-976004 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T152055 NZ_CP065946:1247777-1248265 [Pectobacterium parmentieri]
ATGATTTCCGCCCCTGAGCCACTCCGCGCCGAACATTTACTATCTTCGTTCTGTTGCGGCGTGGAGTCCATGGACAACTG
GCTAAAACAACGGGCGATGAAAAATCAGGTCACTGGAGCATCCCGGACTTTCGTCAGTTGCGATGACTCAAAAGTGTTGG
CGTATTATTCATTGGCTTCCAGTGCTGTAGCAACGAATGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTCGTGGTGTTGGGTCGTCTGGCGGTAGATCAATCGTTACATGGTCATGGTGTTGGCCGAGCACTGGTACGGGATGC
TGGGCTAAGAGTGATTCAGGTTGCTAATACTATCGGCATTCGCGGGATATTGGTTCATGCGCTGTCCGACGAAGCTCGAG
AGTTTTACCTACGAGTTGGGTTTGAGCCTTCACCGATTGACCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTGGGG
AGTTTGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT152055 WP_000581941.1 NZ_CP050036:975421-975669 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT152055 NZ_CP065946:1247487-1247780 [Pectobacterium parmentieri]
ATGCCAGCGACGAACAGCGTTTCAGTTAAACGCGAAACATTGAATTTACGCATCAAACCCGCCGAACGCGATCTGATTGA
TCGTGCTGCGAAGGCCAGAGGGAAAAATCGCACTGATTTTGTCCTTGAGGCCGCGCGTGCTGCCGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATTATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGTTTGGATCAGGCCCCTGCACCAAAT
GCCGCGCTGCGAAAAACTATGCAAACGCCAGCACCGTGGGAACAGAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References