Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 834407..835061 Replicon chromosome
Accession NZ_CP050036
Organism Escherichia coli strain 58-3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag G9P49_RS04055 Protein ID WP_000244777.1
Coordinates 834654..835061 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G9P49_RS04050 Protein ID WP_000354046.1
Coordinates 834407..834673 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P49_RS04025 829576..830319 + 744 WP_000951962.1 SDR family oxidoreductase -
G9P49_RS04030 830376..831809 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
G9P49_RS04035 831854..832165 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G9P49_RS04040 832329..832988 + 660 WP_000250274.1 hemolysin III family protein -
G9P49_RS04045 833184..834164 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G9P49_RS04050 834407..834673 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G9P49_RS04055 834654..835061 + 408 WP_000244777.1 toxin CptA Toxin
G9P49_RS04060 835101..835622 - 522 WP_001055874.1 flavodoxin FldB -
G9P49_RS04065 835734..836630 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G9P49_RS04070 836655..837365 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G9P49_RS04075 837371..839104 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T152054 WP_000244777.1 NZ_CP050036:834654-835061 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T152054 NZ_CP065946:1239553-1239951 [Pectobacterium parmentieri]
ATCAGGATTTTCAAGTCAGCACTGATTCGTCAGCAATTGAGCCAGCAAGAGCTTGACGCTTTGGCGGCGGATTTTCTGTC
CTATAAAAAGGATGGCGTGTTGCCAGATACCTTTGGTCGTGATGCACCCTATGATGATGACCGCACCTATCCTTTGGTAA
AAGAGGAGCAGGTTGCCCACATTCATCTTGCCGATGCGGATGCCCCTTTCCCTAAGTTTTTACGTCAGTTCAAACGAACC
AGCGATCAGGCCCATTTGGTATACTGCCAAGGTGCGATGGATCCCGATGCCTACCTGCTGATTATCATCCTGAAACCCGA
AGCGCATAAAATGGCGCGCAACAATAACCATATGCATAAGATCGGGATGATGGCTGAAGCGTTCAGGATGAAGCATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT152054 WP_000354046.1 NZ_CP050036:834407-834673 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT152054 NZ_CP065946:1239257-1239550 [Pectobacterium parmentieri]
ATGGAACGCATTCTCGCAGAAAAATCTATCAACATCACCGAGTTAAGAAAGAACCCGGCGAAATATTTTATTGATGAACC
GGTTGCTGTTCTCTCCAACAACCGTCCCGCTGGCTACATGGTCAGTGCCAAGGTGTTTGAAGAGCTGATCGATCTGTTGG
AAGAGAAGCAAGGCAGGGTTCATACCGCTGCGCGGTTCAGGCCAACGGCTGAACGTTTAAGTGATATTGCGGATAGCGGG
CAGAAACTGCTGCAAAACGCCTCTGACAAGGATCTGACCGAGTTCACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References