Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4212775..4213272 Replicon chromosome
Accession NZ_CP050031
Organism Escherichia coli strain 33-6

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G9P48_RS20330 Protein ID WP_001173343.1
Coordinates 4212775..4212948 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G9P48_RS20335 Protein ID WP_000398619.1
Coordinates 4212976..4213272 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P48_RS20320 4209440..4210897 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
G9P48_RS20325 4211134..4212651 + 1518 WP_001295074.1 lysine--tRNA ligase -
G9P48_RS20330 4212775..4212948 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G9P48_RS20335 4212976..4213272 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G9P48_RS20340 4213499..4213771 - 273 WP_000405647.1 N-acetyltransferase -
G9P48_RS20345 4213783..4214013 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
G9P48_RS20350 4214194..4215825 + 1632 WP_001216477.1 sensor histidine kinase -
G9P48_RS20355 4215822..4216541 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T152050 WP_001173343.1 NZ_CP050031:c4212948-4212775 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T152050 NZ_CP065939:3020931-3021110 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTGACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATAA
AAAAACAGGCGGGGCTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT152050 WP_000398619.1 NZ_CP050031:c4213272-4212976 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT152050 NZ_CP065939:3021132-3021542 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGTTTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATTGAAAGCGTACGTGAAGCGATCGAGGCTCACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCAGAAGCAACGACCGTCGAAAACTGGCTTTCTGATCCGGAGTACGCCGGTGCGGTCTGGGCCTTGGTAGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATTAATGTGACGCTTCCCTCATTGCTGATCCGTCGTATCGATCA
GTTCGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCCTGTCTCGTGTCGCGGCAGACAAAGTAATTGGACGAG
AAAAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References