Detailed information of TA system TA152045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2460664..2461302
• Replicon: chromosome
• Accession: NZ_CP050031
• Organism: Escherichia coli strain 33-6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: G9P48_RS11920
• Protein ID: WP_000813794.1
• Coordinates: 2461126..2461302 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G9P48_RS11915
• Protein ID: WP_001270286.1
• Coordinates: 2460664..2461080 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P48_RS11895	2455816..2456757	-	942	WP_001251313.1	ABC transporter permease	-
G9P48_RS11900	2456758..2457771	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
G9P48_RS11905	2457789..2458934	-	1146	WP_182116710.1	ABC transporter substrate-binding protein	-
G9P48_RS11910	2459179..2460585	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
G9P48_RS11915	2460664..2461080	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G9P48_RS11920	2461126..2461302	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G9P48_RS11925	2461524..2461754	+	231	WP_000494244.1	YncJ family protein	-
G9P48_RS11930	2461846..2463807	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G9P48_RS11935	2463880..2464416	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
G9P48_RS11940	2464502..2465683	+	1182	WP_182116711.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T152045 WP_000813794.1 NZ_CP050031:c2461302-2461126 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T152045 NZ_CP065939:666314-666655 [Enterobacter hormaechei]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTTGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCTTATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT152045 WP_001270286.1 NZ_CP050031:c2461080-2460664 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT152045 NZ_CP065939:665952-666293 [Enterobacter hormaechei]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTAGCTGACCGGGTCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGGAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Toxin

• PDB: 6HPB