Detailed information of TA system TA152043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2304590..2305116
• Replicon: chromosome
• Accession: NZ_CP050031
• Organism: Escherichia coli strain 33-6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: G9P48_RS11155
• Protein ID: WP_000323025.1
• Coordinates: 2304829..2305116 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: G9P48_RS11150
• Protein ID: WP_000534858.1
• Coordinates: 2304590..2304829 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P48_RS11125	2299926..2300282	+	357	WP_182116904.1	class I SAM-dependent methyltransferase	-
G9P48_RS11130	2300893..2301179	+	287	Protein_2175	hypothetical protein	-
G9P48_RS11135	2301948..2303288	+	1341	WP_000589013.1	ISNCY family transposase	-
G9P48_RS11140	2303725..2304057	-	333	WP_001301033.1	protein FlxA	-
G9P48_RS11145	2304260..2304565	-	306	WP_071593935.1	hypothetical protein	-
G9P48_RS11150	2304590..2304829	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
G9P48_RS11155	2304829..2305116	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
G9P48_RS11160	2305188..2305343	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
G9P48_RS11165	2305560..2305811	+	252	WP_001463433.1	hypothetical protein	-
G9P48_RS11170	2305878..2306156	+	279	WP_001515373.1	hypothetical protein	-
G9P48_RS11175	2306158..2307207	+	1050	WP_182116905.1	DUF968 domain-containing protein	-
G9P48_RS11180	2307225..2307602	+	378	WP_001204787.1	antitermination protein	-
G9P48_RS11185	2307758..2308282	-	525	WP_000780584.1	lipocalin family protein	-
G9P48_RS11190	2308475..2309434	+	960	WP_000592549.1	DUF523 and DUF1722 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2278958..2329394	50436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T152043 WP_000323025.1 NZ_CP050031:2304829-2305116 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T152043 NZ_CP065939:c298680-298378 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT152043 WP_000534858.1 NZ_CP050031:2304590-2304829 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT152043 NZ_CP065939:c298371-298084 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8