Detailed information of TA system TA152027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 689515..691271
• Replicon: chromosome
• Accession: NZ_CP050021
• Organism: Burkholderia thailandensis strain BPM

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q2T555
• Locus tag: G9462_RS19625
• Protein ID: WP_009900384.1
• Coordinates: 690366..691271 (-)
• Length: 302 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q2T556
• Locus tag: G9462_RS19620
• Protein ID: WP_009897321.1
• Coordinates: 689515..690369 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9462_RS19600	684735..686225	-	1491	WP_043296718.1	aldehyde dehydrogenase	-
G9462_RS19605	686247..687062	-	816	WP_009897315.1	aspartate dehydrogenase	-
G9462_RS19610	687055..688728	-	1674	WP_009897317.1	thiamine pyrophosphate-binding protein	-
G9462_RS19615	688721..689518	-	798	WP_009897318.1	SDR family oxidoreductase	-
G9462_RS19620	689515..690369	-	855	WP_009897321.1	IclR family transcriptional regulator	Antitoxin
G9462_RS19625	690366..691271	-	906	WP_009900384.1	alpha/beta fold hydrolase	Toxin
G9462_RS19630	691288..691815	-	528	WP_009897327.1	cupin domain-containing protein	-
G9462_RS19635	691854..692624	-	771	WP_009897331.1	SDR family oxidoreductase	-
G9462_RS19640	692621..693688	-	1068	WP_009897333.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
G9462_RS19645	693723..694034	-	312	WP_009897334.1	hypothetical protein	-
G9462_RS19650	694036..694353	-	318	WP_009897336.1	non-heme iron oxygenase ferredoxin subunit	-
G9462_RS19655	694788..695876	+	1089	WP_025369849.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (54-285)

Antitoxin

• Transcription regulator IclR, C-terminal (143-265)

Sequences

Toxin

Protein

• Length: 302 a.a.
• Molecular weight: 30752.67 Da
• Isoelectric Point: 8.4768

>T152027 WP_009900384.1 NZ_CP050021:c691271-690366 [Burkholderia thailandensis]
MSAISTTTTDASATPFAALLERFAERRAAAGGARTIGYREAGAAYAAHAGRDPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGVSSRVAADSPVASDYAASLAAWLDALRIERCVLVGHSLGAIVAGAFARQAGERLSGLLLISPAGGYGGAPA
DVRAERRDSRLALLASLGPQGLAAERSANMLSAQASDAARAWVRWNMARIVPAGYAQATHLLANADLASDLAHYRGRVAV
AVGADDTITPPAACERIARAAGAGLQVIPRAGHAGYVEAPDAYNALIDAFCRQCDGSRGAA

Nucleotide

• Length: 906 bp

>T152027 NZ_CP065925:2244946-2245284 [Staphylococcus pseudintermedius]
ATGAGTATTAAACAATTTGATATCATTTACATTGATTTAAATCCAACTCGCGGTCGTGAAAAACAAAACATAAGACCTTG
TTTAGTGATCAATAACCAAATGATGATTGACGGCACTGAACTTGTATGGGTTGTACCTATCATAAATCGTGAGTTGCGTT
ATCCAACCGATATAGAACTTAAAACCAAAAAAGGGCTGGTATCTGGCGTGATAGATTCGATTCAAATACGTGCTTTAGAC
TTAAACGCTCGCCAACATCACTATAAAGATGCATTACAAGACAATCTAAAAAGTAAAGTTTTAAAAGCAATACAAACATA
TTTAAATCCAAACTACTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31148.57 Da
• Isoelectric Point: 6.9978

>AT152027 WP_009897321.1 NZ_CP050021:c690369-689515 [Burkholderia thailandensis]
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIEGLRDATGFTAHIVIRDGRDVVFVAKAQNAGHAFGSVRVNVGTRLPAHATTHGHV
LMGDLTLAELRTLYPERALERVTKATPETVDALFDVIRADARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDASLVADGLIDKVVRAAAELSRRLNYRSDDENTYLKALGLR

Nucleotide

• Length: 855 bp

>AT152027 NZ_CP065925:2244749-2244949 [Staphylococcus pseudintermedius]
ATGAGTTTAAGTAAAAAAACGATTCAATCCGTTGATTTTGAGATTGGCGATACAGTTGAAGTACAAAAAATAAATGGCGG
GCTGTTCATTACTAAAAAAGAAGTATCTATTGAAGATAGTATCAAAAACTTCTTCCAAAATGGTGGTAAGTATCAAGAAT
CAGAAGTAGAGTTCGGCGAAAGTGTGGGTCGTGAAATATGA

Structures

Toxin

• AlphaFold DB: Q2T555

Antitoxin

• AlphaFold DB: Q2T556