Detailed information of TA system TA152009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1165728..1166349
• Replicon: chromosome
• Accession: NZ_CP050013
• Organism: Serratia marcescens strain BP2

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A8B4G7F9
• Locus tag: HAP49_RS05510
• Protein ID: WP_004940313.1
• Coordinates: 1165728..1165931 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A2X2G5J9
• Locus tag: HAP49_RS05515
• Protein ID: WP_004940312.1
• Coordinates: 1165981..1166349 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP49_RS05480	1161427..1161765	+	339	WP_004940327.1	P-II family nitrogen regulator	-
HAP49_RS05485	1161802..1163088	+	1287	WP_004940322.1	ammonium transporter AmtB	-
HAP49_RS05490	1163179..1164042	-	864	WP_015376854.1	acyl-CoA thioesterase II	-
HAP49_RS05495	1164274..1164765	+	492	WP_015376855.1	YbaY family lipoprotein	-
HAP49_RS05500	1164830..1165144	-	315	WP_166444913.1	MGMT family protein	-
HAP49_RS05510	1165728..1165931	-	204	WP_004940313.1	hemolysin expression modulator Hha	Toxin
HAP49_RS05515	1165981..1166349	-	369	WP_004940312.1	Hha toxicity modulator TomB	Antitoxin
HAP49_RS05520	1166508..1166861	-	354	WP_004940311.1	hypothetical protein	-
HAP49_RS05525	1167264..1167977	+	714	WP_118892386.1	ABC transporter ATP-binding protein	-
HAP49_RS05530	1167974..1168831	+	858	WP_044031827.1	metal ABC transporter permease	-
HAP49_RS05535	1168857..1169735	+	879	WP_118892385.1	metal ABC transporter substrate-binding protein	-
HAP49_RS05540	1169843..1169983	-	141	WP_004940304.1	type B 50S ribosomal protein L36	-
HAP49_RS05545	1169996..1170250	-	255	WP_004940303.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8107.37 Da
• Isoelectric Point: 6.9756

>T152009 WP_004940313.1 NZ_CP050013:c1165931-1165728 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Nucleotide

• Length: 204 bp

>T152009 NZ_CP065918:2391233-2391691 [Yersinia pestis EV NIIEG]
ATGACGCTATACCGCATTGTTATGCGCCGCTATCTTGCATCGGCATGGACTGGTTACGGCGCTGAAATCTATGGTGGTCG
TTGGAATCATAAAGGCCATGCTGCCATCTATTTAGCCAGCAGTATTTCATTGGCTATGCTCGAAACACTGGCACATATTC
AGGACAGTTCAACACTCAGCGAATTTGAATTATTCCAAATTGAAATCAGTGACAGCAGTATTATGTTGCTACAGCCGCAG
GACTGGCCCACGGATTGGCGTAGCGATCCGGCACCGGTGGCAACGATGGATGTCGGCACCGAGTGGCTGGAATCAGAATC
CTCGGTAGGCTTACTGGTCCCGTCCACGTTGGTGCCGTCAGAAAACAACCTACTGGTCAATCCTCATCACAAAGATTTTC
CAGCGTGTTTGGCCAGCGTCAAACCGCTCTCTTTTGTCTTTGATCCACGGTTAAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14261.95 Da
• Isoelectric Point: 4.4314

>AT152009 WP_004940312.1 NZ_CP050013:c1166349-1165981 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Nucleotide

• Length: 369 bp

>AT152009 NZ_CP065918:2390725-2391171 [Yersinia pestis EV NIIEG]
ATGAGAGCGTACCGCCCAAACCCCGCGACTAAAATGGGAACGCTTTGGCGTGAAATTGGCTTACCCGCCAGCCGCGGCAC
GATATTGGTCGATAGTATCAAAATGGGGTTTTCTGTTGATGTTATCGACAGCATTCATTTATGGGCATCCATGCCTAAAG
CTGAAATATTACGTGCTACCGGTATCCCCAGCCGTAGCCTGACTCGCCGCCGCACACATGATGGCCGTTTTACGCCTGAA
GAAAGCGAGAGAATCGCACGTTTTGTCCGCGTAATGGATGCCGCTGTCGATTTGTTCGGCGGCGATAAAGGTAAAGCCAT
TACCTGGATGTCCACGCCCATCAAAGGATTGGGGTACCGCAGCCCAGACTCCTTACTGGAAACCGAAACTGGCGCACTGG
AAGTGTGTGATTTGATTGGTCGTTTGGAACACGGCGTCTTTACTTAA

Structures

Toxin

• AlphaFold DB: A0A8B4G7F9

Antitoxin

• AlphaFold DB: A0A2X2G5J9