Detailed information of TA system TA152008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 45852..46495
• Replicon: plasmid unnamed2
• Accession: NZ_CP050011
• Organism: Citrobacter sp. Y3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HAP28_RS25860
• Protein ID: WP_166444358.1
• Coordinates: 46079..46495 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HAP28_RS25855
• Protein ID: WP_166444357.1
• Coordinates: 45852..46082 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP28_RS25835	41473..41703	+	231	WP_024553479.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HAP28_RS25840	41700..42116	+	417	WP_024553480.1	type II toxin-antitoxin system VapC family toxin	-
HAP28_RS25850	43522..45486	+	1965	WP_166444370.1	NERD domain-containing protein	-
HAP28_RS25855	45852..46082	+	231	WP_166444357.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HAP28_RS25860	46079..46495	+	417	WP_166444358.1	type II toxin-antitoxin system VapC family toxin	Toxin
HAP28_RS25865	46683..47585	-	903	WP_061549446.1	LysR family transcriptional regulator	-
HAP28_RS25870	47709..49100	+	1392	WP_061549445.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89705	89705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15160.68 Da
• Isoelectric Point: 8.5291

>T152008 WP_166444358.1 NZ_CP050011:46079-46495 [Citrobacter sp. Y3]
VNKIYMLDTNICSFIMREQPETVLKRLEQAVLRGHRIVVSVITYSEMRFGATGPKASPRHVQLVDALCARLDAILPWDRT
AVDATTKIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLILEDWVN

Nucleotide

• Length: 417 bp

>T152008 NZ_CP065918:c1906440-1906258 [Yersinia pestis EV NIIEG]
GTGAAAAGTTCGGAGCTGATAAAACTGCTCGAAACAAATGGTTGGAAGTTGATGAGAGTTAAAGGCAGTCATCATCAATT
CAAACATCCTGATTTCGCGGCAGTCATAACAGTACCGCATCCCAAAAAGGATGTTAAACCGGGAACCCTCCGGCAAATTC
TGAAAGATGCAAACCTTAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8638.74 Da
• Isoelectric Point: 4.6017

>AT152008 WP_166444357.1 NZ_CP050011:45852-46082 [Citrobacter sp. Y3]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRATWGSFAQLEKADPGFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT152008 NZ_CP065918:c1906213-1905794 [Yersinia pestis EV NIIEG]
ATGGCTATCTATCCCGCCTATGTTCACGTTGATAATGATGGCTCGGCAAGCGGTTATTTCCCCGATGTCAAAGGCTGTAT
TTTTGCCATCAATGCCGGGGAGGATTTATTTGCAGAAGCATCAAGCGCCCTGGATGCACACTTTGAAGCTTTGGTGTCTG
AAGGTATCGAAATTCCTGAAGCTCATGACATGCCTTACCACGTTTACCGTAACCCATGCGATTACGCTGATGGCGGACAG
TGGTATAACGTGAATATTGATATGTCGAAATATGATGGAAAAGTTGAACGAATTAACGTCACTCTTCCGCACCGGCTGAT
TCATCAAATCGATACTATTGTTAAAGTGCGTCCCGAGTATGCCAGCCGAAGCAATTTTCTAGCCGAAGCAGCACGTAAAG
AATTACAGAAATTGGCGTAA