Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 9661..10187 Replicon plasmid unnamed2
Accession NZ_CP050011
Organism Citrobacter sp. Y3

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HAP28_RS25640 Protein ID WP_000323025.1
Coordinates 9661..9948 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HAP28_RS25645 Protein ID WP_000534858.1
Coordinates 9948..10187 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP28_RS25600 5479..5814 - 336 WP_166444336.1 hypothetical protein -
HAP28_RS25605 5857..6648 - 792 WP_166444337.1 N-6 DNA methylase -
HAP28_RS25610 6689..6835 - 147 WP_047353114.1 hypothetical protein -
HAP28_RS25615 6923..7384 - 462 WP_166444338.1 DUF1419 domain-containing protein -
HAP28_RS25620 7462..7668 - 207 WP_166444339.1 hypothetical protein -
HAP28_RS25625 7730..8044 - 315 WP_166444340.1 hypothetical protein -
HAP28_RS25630 8669..9154 + 486 WP_166444341.1 cytoplasmic protein -
HAP28_RS25635 9431..9589 - 159 WP_014063856.1 type I toxin-antitoxin system Hok family toxin -
HAP28_RS25640 9661..9948 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HAP28_RS25645 9948..10187 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HAP28_RS25650 10450..11373 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
HAP28_RS25655 11573..12145 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HAP28_RS25660 12788..14128 - 1341 WP_000589001.1 ISNCY family transposase -
HAP28_RS25665 14438..14632 - 195 WP_166444367.1 hypothetical protein -
HAP28_RS25670 14674..14904 - 231 WP_166444342.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..89705 89705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T152005 WP_000323025.1 NZ_CP050011:c9948-9661 [Citrobacter sp. Y3]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T152005 NZ_CP065912:3029-3355 [Paracoccus marcusii]
GTGAAGCGCGGGGATCTGGTGACGGTCGCCTTGCAGGGCGATTACGGCAAACCGCGACCGGCGCTTGTGATCCAGTCCGA
TCAGTTTCCCGGCACGGCCTCCGTTGTGGTCCTGCCGGTCACCTCGACGCTGATGGATGCGCCCTTGCTGCGCCTGACAG
TCGAACCCGACGCCGGGAACCGCCTGCGGGCCACGTCGCAAATCATGCTGGATAAGCCCATGACCGTGAAGGCCGACAAG
ATCGGCCCTGCCTTCGGGTCGCTCGATGACGTGGCCATGGTCAGCGTCAGCCGTTCTCTGGCCCTGTTTCTCGGACTGGC
AGGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT152005 WP_000534858.1 NZ_CP050011:c10187-9948 [Citrobacter sp. Y3]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT152005 NZ_CP065912:2814-3032 [Paracoccus marcusii]
ATGCCCACCCCAGTTTCCGAACGTGTCCAGAAGCGCCGCGCGGCTCTGCGGGCCGCTGGACTGCGCCCTGTCCAGATCTG
GGTGCCTGACACCCGACGCCCCGGCTTTGCCGCCGAGTGCCGCCGTCAGGCCGCTCTGGTGGCCGAAGCTGACCGGACCG
ATACCGACATGCAGGATTTCATGGATGCCGCCCTGGCGGATCTGGACGACTGGCAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References