Detailed information of TA system TA152002

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4486757..4487436
• Replicon: chromosome
• Accession: NZ_CP050009
• Organism: Citrobacter sp. Y3

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HAP28_RS21755
• Protein ID: WP_109739503.1
• Coordinates: 4486757..4487098 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HAP28_RS21760
• Protein ID: WP_109739504.1
• Coordinates: 4487119..4487436 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP28_RS21730	4482487..4483362	+	876	WP_000774153.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
HAP28_RS21735	4483411..4483701	+	291	WP_044264508.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
HAP28_RS21740	4483712..4484458	+	747	WP_109739500.1	epimerase	-
HAP28_RS21745	4484778..4485575	-	798	WP_109739501.1	helix-turn-helix transcriptional regulator	-
HAP28_RS21750	4485808..4486641	-	834	WP_109739502.1	DUF4942 domain-containing protein	-
HAP28_RS21755	4486757..4487098	-	342	WP_109739503.1	TA system toxin CbtA family protein	Toxin
HAP28_RS21760	4487119..4487436	-	318	WP_109739504.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HAP28_RS21765	4487455..4487676	-	222	WP_109739505.1	DUF987 domain-containing protein	-
HAP28_RS21770	4487685..4488161	-	477	WP_109739506.1	RadC family protein	-
HAP28_RS21775	4488177..4488635	-	459	WP_109739507.1	antirestriction protein	-
HAP28_RS21780	4488732..4488971	-	240	WP_109739508.1	DUF905 domain-containing protein	-
HAP28_RS21785	4489049..4489522	-	474	WP_109739509.1	hypothetical protein	-
HAP28_RS21790	4489544..4490383	-	840	WP_109739510.1	hypothetical protein	-
HAP28_RS21795	4490422..4491123	-	702	WP_109739511.1	WYL domain-containing protein	-
HAP28_RS21800	4491340..4492161	-	822	WP_109739512.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4484778..4507281	22503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12885.04 Da
• Isoelectric Point: 9.9572

>T152002 WP_109739503.1 NZ_CP050009:c4487098-4486757 [Citrobacter sp. Y3]
MKILPATISRAAKPCLPPVAVWQMLLTRLLEKHYGLTLNDTPFSDEIVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNAVR

Nucleotide

• Length: 342 bp

>T152002 NZ_CP065904:7988-8314 [Paracoccus marcusii]
GTGAAGCGCGGTGATCTGGTGACGGTCGCCTTGCAGGGTGATTATGGCAAACCGCGCCCGGCGCTTGTGATCCAGTCGGA
TCAGTTCTCCGGCACGGCATCCGTTGTGGTCCTGCCGGTAACCTCGACGCTGGTGGATGCGCCTCTGCTGCGTCTGACGG
TCGAACCCGAAGCCGGGAACGGCCTGCGGACCACGTCGCAGATCATGTTGGATAAGCCCATGACCGTGAAGGCCGACAAG
ATCGGCCCTGCTTTCGGATCGCTCGATGACGTCGCCATGGTCAGCATCAGCCGCTCTCTGGCCCTGTTTCTCGGACTGGC
AGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11796.40 Da
• Isoelectric Point: 6.9367

>AT152002 WP_109739504.1 NZ_CP050009:c4487436-4487119 [Citrobacter sp. Y3]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPHFISQIESMLTTYELNPRHAHCVTLCH
NGFTCEADTLGSCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT152002 NZ_CP065904:7773-7991 [Paracoccus marcusii]
ATGCCAACCCCAGTTTCCGAACGTGTCCAGAAGCGCCGCGCGGCTCTGCGGGCCGCTGGACTGCGCCCTGTCCAGATCTG
GGTGCCGGACACCCGACGCCCCGGCTTTGCCGCCGAGTGCCGCCGTCAAGCCGCTCTGGTGGCTGAAGCTGACCGAACCG
ATACCGACATGCAGAATTTTATGGATGCCGCCCTGACGGACCTGGACGACTGGCAGTGA