Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1474901..1475521 Replicon chromosome
Accession NZ_CP050009
Organism Citrobacter sp. Y3

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag HAP28_RS06975 Protein ID WP_001280991.1
Coordinates 1474901..1475119 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag HAP28_RS06980 Protein ID WP_042324455.1
Coordinates 1475147..1475521 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP28_RS06945 1470554..1471150 + 597 WP_044258943.1 membrane protein -
HAP28_RS06950 1471233..1471586 + 354 WP_043001561.1 DUF1428 family protein -
HAP28_RS06955 1471626..1473176 - 1551 WP_109739954.1 EAL domain-containing protein -
HAP28_RS06960 1473399..1473539 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HAP28_RS06965 1473589..1474059 - 471 WP_043001559.1 YlaC family protein -
HAP28_RS06970 1474173..1474724 - 552 WP_044258934.1 maltose O-acetyltransferase -
HAP28_RS06975 1474901..1475119 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
HAP28_RS06980 1475147..1475521 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
HAP28_RS06985 1476032..1479181 - 3150 WP_043001557.1 multidrug efflux RND transporter permease subunit -
HAP28_RS06990 1479204..1480397 - 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T151988 WP_001280991.1 NZ_CP050009:c1475119-1474901 [Citrobacter sp. Y3]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T151988 NZ_CP065866:c5425662-5425384 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT151988 WP_042324455.1 NZ_CP050009:c1475521-1475147 [Citrobacter sp. Y3]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT151988 NZ_CP065866:c5425372-5425067 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References