Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4663754..4664408 Replicon chromosome
Accession NZ_CP049979
Organism Escherichia coli strain IBS28

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HAP53_RS22290 Protein ID WP_000244772.1
Coordinates 4663754..4664161 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HAP53_RS22295 Protein ID WP_000354046.1
Coordinates 4664142..4664408 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP53_RS22270 4659711..4661444 - 1734 WP_000813214.1 single-stranded-DNA-specific exonuclease RecJ -
HAP53_RS22275 4661450..4662160 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HAP53_RS22280 4662185..4663081 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HAP53_RS22285 4663193..4663714 + 522 WP_001055874.1 flavodoxin FldB -
HAP53_RS22290 4663754..4664161 - 408 WP_000244772.1 protein YgfX Toxin
HAP53_RS22295 4664142..4664408 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HAP53_RS22300 4664651..4665631 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HAP53_RS22305 4665708..4666367 - 660 WP_000250274.1 hemolysin III family protein -
HAP53_RS22310 4666531..4666842 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
HAP53_RS22315 4666887..4668320 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HAP53_RS22320 4668377..4669120 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T151961 WP_000244772.1 NZ_CP049979:c4664161-4663754 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T151961 NZ_CP065848:5200228-5200407 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCCGACGCTGGCTGAGGGCACAAGGTGTGACCTTCGAAGCCGGCAAGGGCAGTCACTTCAAGGT
GACCGCCCCGAACGGTAAGACAACGACCTTCGCAGATCACGGCGCCAAAGAAATGCCGGAGCCGACACGCAAGGCGATCA
TCAAGCAACTGGGGCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT151961 WP_000354046.1 NZ_CP049979:c4664408-4664142 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT151961 NZ_CP065848:5200448-5200879 [Pseudomonas aeruginosa]
ATGTACAACTTCGCAATCCGTTTCGAGCAAGACGACAGCGCTCCTGGCGTTGCCGTTTTCTGTCGAGACCTTCCAGAGCT
GAACAGCTACGGAAACACCGTCGAGCACGCACTCGGCGAGGCCGTTGATGCCATCGAAACCACATTGTCGCTCTACGTTG
ATCAGCGCCGACCAATTCCAGCGGCTAGCGCTCCAGAACCAGAAGAGCACGTAGTGCATCTTCCAGCGGTGACCGTCGCG
AAGATCGCTCTATGGAACGAGATGATGCGGCGCGACATGCGGAAGGCTGATCTATGCCGGGCTCTAGGCGTCGCCCAAGT
ACAGGTCGACCGCCTCGTCGACTTTCTGCATACCTCGAAGATGGAGCAGTTGGAGTCCGCGTTAGGGGTCTTAGGCTTGC
GTCTTTTCGTGAGTGTGCAGCAGGCTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References