Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4527491..4528074 Replicon chromosome
Accession NZ_CP049979
Organism Escherichia coli strain IBS28

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag HAP53_RS21670 Protein ID WP_000254745.1
Coordinates 4527491..4527826 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HAP53_RS21675 Protein ID WP_000581937.1
Coordinates 4527826..4528074 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP53_RS21650 4522960..4523322 + 363 WP_000034929.1 hypothetical protein -
HAP53_RS21655 4523378..4524676 - 1299 WP_000036723.1 phosphopyruvate hydratase -
HAP53_RS21660 4524764..4526401 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HAP53_RS21665 4526629..4527420 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HAP53_RS21670 4527491..4527826 - 336 WP_000254745.1 endoribonuclease MazF Toxin
HAP53_RS21675 4527826..4528074 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HAP53_RS21680 4528152..4530386 - 2235 WP_000226815.1 GTP pyrophosphokinase -
HAP53_RS21685 4530434..4531735 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T151960 WP_000254745.1 NZ_CP049979:c4527826-4527491 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T151960 NZ_CP065848:c5068938-5068534 [Pseudomonas aeruginosa]
ATGCTGAAGTACATGCTTGATACCAACATGTGCATTTTCACGATCAAGAATCGTCCTGAGCAGGTGCGGGAAGCCTTCAA
GCGCCATTCAGGGCAGCTGAGCATCAGCACTGTCACCCTGATGGAGCTTATTTACGGCGCCGAGAAGTCCGCTAATCCCG
AGCGTAATCTGGCGGACGTTGAAGGTTTCGCTGCGCGCCTGGAAGTACTGCCTTACGACGCTCAGGCAGCGGCTCATTCC
GGGCAATTGCGAGCTGAGTTGGCTCGTATCGGCAAGCCTATTGGCCCGTATGATCAGATGATTGCAGGACACGCACGTGC
CCAGGGACTGATTCTGGTCACGAATAATCTTCGTGAATTCGAGCGAGTACCGGGTTTGCGCGTCGAGGATTGGGTGAGCG
CCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT151960 WP_000581937.1 NZ_CP049979:c4528074-4527826 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT151960 NZ_CP065848:c5069168-5068938 [Pseudomonas aeruginosa]
ATGGAGCAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAGGCGGTGCGTTTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTGGTAGTTGGTCGAGCCCGAATCATCACGCCGGCAGGCGAGGCCTGGGACGTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAGCCTGCAGATCAAGAGCGGGATGCGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References