Detailed information of TA system TA151959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4239207..4239832
• Replicon: chromosome
• Accession: NZ_CP049979
• Organism: Escherichia coli strain IBS28

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HAP53_RS20265
• Protein ID: WP_000911330.1
• Coordinates: 4239207..4239605 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HAP53_RS20270
• Protein ID: WP_000450524.1
• Coordinates: 4239605..4239832 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP53_RS20245	4235085..4235285	+	201	WP_000383836.1	YpfN family protein	-
HAP53_RS20250	4235395..4236093	-	699	WP_000679823.1	esterase	-
HAP53_RS20255	4236167..4238182	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
HAP53_RS20260	4238197..4239060	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
HAP53_RS20265	4239207..4239605	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HAP53_RS20270	4239605..4239832	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HAP53_RS20275	4239986..4240699	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HAP53_RS20280	4240912..4241946	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HAP53_RS20285	4241963..4242841	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HAP53_RS20290	4242987..4243559	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HAP53_RS20295	4243559..4244029	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T151959 WP_000911330.1 NZ_CP049979:c4239605-4239207 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T151959 NZ_CP065848:c5066427-5066134 [Pseudomonas aeruginosa]
GTGCCACGATTGATTGTTACTGAAGGCGCGGCGAAGGGCTTGGAGCGTTGCCGGCGATTCCTCTCAGACAAAGATCCGCA
GGTCGCGCGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCGGAGCTGCGTGAGCTCATCATCGAATTTGGTGACTCAGGTTACGTAGCGTTGTACCGCTATGAGCGTGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT151959 WP_000450524.1 NZ_CP049979:c4239832-4239605 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT151959 NZ_CP065848:c5066687-5066415 [Pseudomonas aeruginosa]
ATGGCCACTTCCATCAAAATTGATGACGAGCTGAAAGGCCGCATTCAGCAGTTGGCGAGCCAACGCCAGCGCTCGTCTCA
CTGGATCATGCGCGAGGCAATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAGCAGGAAGCCCTGGCAT
CGTGGGCGGCATATCAGGAAACCGGACGGCATTTGACCAGCCAAGAAACTCGGACCTGGCTCGGTAGCTGGGGAACAGAT
ACTGAGGTAGAGCTTCCCAAGTGCCACGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33