Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 725119..725919 Replicon chromosome
Accession NZ_CP049979
Organism Escherichia coli strain IBS28

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag HAP53_RS03285 Protein ID WP_000342449.1
Coordinates 725392..725919 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag HAP53_RS03280 Protein ID WP_001277108.1
Coordinates 725119..725385 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP53_RS03260 720777..721445 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HAP53_RS03265 721438..722496 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
HAP53_RS03270 722741..723595 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HAP53_RS03275 723866..724969 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HAP53_RS03280 725119..725385 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
HAP53_RS03285 725392..725919 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
HAP53_RS03290 725916..726299 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
HAP53_RS03295 726723..727832 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HAP53_RS03300 727880..728806 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HAP53_RS03305 728803..730080 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
HAP53_RS03310 730077..730844 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T151933 WP_000342449.1 NZ_CP049979:725392-725919 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T151933 NZ_CP065841:c235251-234835 [Klebsiella quasipneumoniae]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT151933 WP_001277108.1 NZ_CP049979:725119-725385 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT151933 NZ_CP065841:c235478-235248 [Klebsiella quasipneumoniae]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References