Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241112..241754 Replicon chromosome
Accession NZ_CP049978
Organism Bacillus sp. RZ2MS9

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BHV55_RS01365 Protein ID WP_000635965.1
Coordinates 241404..241754 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BHV55_RS01360 Protein ID WP_000004570.1
Coordinates 241112..241399 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHV55_RS01335 236429..237391 + 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -
BHV55_RS01340 237384..237956 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
BHV55_RS01345 238050..238409 + 360 WP_070757059.1 holo-ACP synthase -
BHV55_RS01350 238566..239516 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
BHV55_RS01355 239634..240803 + 1170 WP_000390617.1 alanine racemase -
BHV55_RS01360 241112..241399 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BHV55_RS01365 241404..241754 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BHV55_RS01370 241822..243990 + 2169 WP_000426241.1 RNA-binding transcriptional accessory protein -
BHV55_RS01375 244048..244164 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BHV55_RS01380 244360..244818 + 459 WP_000344242.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T151930 WP_000635965.1 NZ_CP049978:241404-241754 [Bacillus sp. RZ2MS9]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T151930 NZ_CP065838:c4696010-4695729 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT151930 WP_000004570.1 NZ_CP049978:241112-241399 [Bacillus sp. RZ2MS9]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT151930 NZ_CP065838:c4696242-4696000 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References