Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 31748..32373 Replicon plasmid pCO_Eco4457-3
Accession NZ_CP049970
Organism Escherichia coli strain ARL09/232

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7QA53
Locus tag G9448_RS25670 Protein ID WP_039023147.1
Coordinates 31975..32373 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7QA08
Locus tag G9448_RS25665 Protein ID WP_039023146.1
Coordinates 31748..31975 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9448_RS25665 31748..31975 + 228 WP_039023146.1 toxin-antitoxin system antitoxin VapB Antitoxin
G9448_RS25670 31975..32373 + 399 WP_039023147.1 tRNA(fMet)-specific endonuclease VapC Toxin
G9448_RS25675 32382..34589 - 2208 WP_039052471.1 type IV conjugative transfer system coupling protein TraD -
G9448_RS25680 34842..35573 - 732 WP_000850422.1 complement resistance protein TraT -
G9448_RS25685 35605..36102 - 498 WP_000605862.1 entry exclusion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..101847 101847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14885.26 Da        Isoelectric Point: 8.2824

>T151929 WP_039023147.1 NZ_CP049970:31975-32373 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARCRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T151929 NZ_CP065838:c4631852-4631511 [Klebsiella quasipneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGACAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8549.62 Da        Isoelectric Point: 4.7513

>AT151929 WP_039023146.1 NZ_CP049970:31748-31975 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAIGRTRLITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT151929 NZ_CP065838:c4632202-4631876 [Klebsiella quasipneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGAAAATTTGGTGACGTCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7QA53

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7QA08

References