Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4428147..4428772 Replicon chromosome
Accession NZ_CP049967
Organism Escherichia coli strain ARL09/232

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag G9448_RS21610 Protein ID WP_000911329.1
Coordinates 4428374..4428772 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag G9448_RS21605 Protein ID WP_000450524.1
Coordinates 4428147..4428374 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9448_RS21580 4423948..4424418 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
G9448_RS21585 4424418..4424990 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
G9448_RS21590 4425136..4426014 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
G9448_RS21595 4426031..4427065 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
G9448_RS21600 4427278..4427991 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
G9448_RS21605 4428147..4428374 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
G9448_RS21610 4428374..4428772 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
G9448_RS21615 4428919..4429782 + 864 WP_001267507.1 neutral zinc metallopeptidase -
G9448_RS21620 4429797..4431812 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
G9448_RS21625 4431886..4432584 + 699 WP_000679812.1 esterase -
G9448_RS21630 4432694..4432894 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T151923 WP_000911329.1 NZ_CP049967:4428374-4428772 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T151923 NZ_CP065834:c292124-291654 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTAATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT151923 WP_000450524.1 NZ_CP049967:4428147-4428374 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT151923 NZ_CP065834:c292564-292121 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTCCTGGGGCAAATCAATGAAGGCCTGGACGGGAAGGTGGCAAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCTTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References