Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3112590..3113257 Replicon chromosome
Accession NZ_CP049967
Organism Escherichia coli strain ARL09/232

Toxin (Protein)


Gene name ypjF Uniprot ID E1IS66
Locus tag G9448_RS15215 Protein ID WP_000856973.1
Coordinates 3112590..3112910 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID E1IS67
Locus tag G9448_RS15220 Protein ID WP_000052843.1
Coordinates 3112931..3113257 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9448_RS15190 3108268..3108561 - 294 WP_000805509.1 hypothetical protein -
G9448_RS15195 3108783..3109601 + 819 WP_000779426.1 lipoprotein NlpA -
G9448_RS15200 3109605..3110528 - 924 WP_000535971.1 carboxylate/amino acid/amine transporter -
G9448_RS15205 3110639..3111823 - 1185 WP_001172876.1 sugar efflux transporter -
G9448_RS15210 3112205..3112351 - 147 WP_001315909.1 hypothetical protein -
G9448_RS15215 3112590..3112910 - 321 WP_000856973.1 TA system toxin CbtA family protein Toxin
G9448_RS15220 3112931..3113257 - 327 WP_000052843.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
G9448_RS15225 3113270..3113740 - 471 WP_001275770.1 DNA repair protein RadC -
G9448_RS15230 3113809..3114546 - 738 Protein_2963 DUF945 domain-containing protein -
G9448_RS15240 3115984..3116994 - 1011 WP_001348677.1 hypothetical protein -
G9448_RS15245 3117056..3117745 - 690 WP_000115588.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-98)

Antitoxin

Antitoxin CbeA

(7-107)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12080.87 Da        Isoelectric Point: 5.5873

>T151919 WP_000856973.1 NZ_CP049967:c3112910-3112590 [Escherichia coli]
MKTQPATTPQAAKPCLSPLAVWQMLLTSLLDQHYGLTLNDTPFSDETVIQKHIEAGITLADAVNFLVERYELVRIDQKGF
SVQDQEPWLTSMDVHRARFKLNVERL

Download         Length: 321 bp

>T151919 NZ_CP065834:228563-229090 [Klebsiella pneumoniae]
GTGATGCAAGAGCTGGCGATAGAGATGATCAACGACGAATTTGACTATGACATAGGCGAATTCGATTGTGGAGAAGAAAC
CTTAAATACATTTTTGAAAGACCATCTCAAGCGGCAGCATGATGGGCAAATTTTAAAAGGTTATGTATTAGTAACCAAAG
AACAGAAGCCTAAGCTGTTAGGCTATTACACATTATCCGGGAGCTGTTTCGAGAGAAAAATGCTCCCGTCAAAAACACAG
CAAAGAAAAATCCCTTATCAGAATGCGCCAAGCATTACTTTGGGACGCTTGGCTATTGACAAATCAATTCAAGGTCAGGG
ATGGGGTGAATTGCTAGTAACTCACGCAATGAATGTCGTCTGGGATGCATCTAAGGCCGTAGGAATATATGGTCTCTTCG
TGGAAGCTCTGAACGATAAAGCTAAGCGCTTCTATACCAGATTAGATTTCATACAGCTCGTTGGTGAGAATAGTAATCTG
CTATTTTACCCGACAAAATCTATTGAAAAAATCTTTACCGAAGAGTAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12305.01 Da        Isoelectric Point: 6.3620

>AT151919 WP_000052843.1 NZ_CP049967:c3113257-3112931 [Escherichia coli]
MSLIPAHEWGLQREIVPRFGARLVQEGNRLHYLADRANLMGNFSDTECFKLNDAFPHFISQMESMLTTGELIPCHHHCVT
LYHNGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 327 bp

>AT151919 NZ_CP065834:228286-228555 [Klebsiella pneumoniae]
ATGTCCGCACTGAGAAAGCAGCGCATAGATCTAAGATTAACTGATGACGACAAAAGCTTAATCGAAGAAGCCGCGGCAAT
GTCAAACCAGACTATTACACAGTTTGTAGTTGCCAGTGCCTCAGAACGTGCAGCGGAAGTGATTGAACAACATCGCAGAA
TGGTTCTAAACGAGGAGTCTTGGAACCTTGTCATGGATGCGATCGCAAACCCTGCTGCGCCGAATGAGCGGCTGAAGCGA
GCAGCGAAGCGTCTACAAAAAATGAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A345ENE3

Antitoxin

Source ID Structure
AlphaFold DB A0A345ENE4

References